Description

3-(3-(3-(Trifluoromethyl)Diazirin-3-Yl)Phenyl)-2,3-Dihydroxypropionic Acid n-Hydroxysuccinimide Ester is a specialized, high-value photoaffinity labeling reagent. This compound is critical for researchers in structural biology and drug discovery, enabling the covalent cross-linking and identification of biomolecular interaction sites. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and biotech companies engaged in proteomics and target identification studies.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalently tagging proteins, nucleic acids, or other biomolecules upon UV light activation to study binding sites and interactions.
• Proteomics Research: Used in activity-based protein profiling (ABPP) and chemoproteomics to identify and characterize enzyme active sites and drug targets.
• Drug Discovery & Development: Facilitates the study of drug-target engagement, off-target effects, and the mechanism of action for novel therapeutic candidates.
• Bioconjugation & Probe Synthesis: The NHS ester moiety allows for efficient conjugation to primary amines, enabling the synthesis of custom molecular probes and affinity tags.
• Structural Biology: Aids in mapping ligand-binding pockets and protein-protein interaction interfaces through cross-linking mass spectrometry.
• Chemical Biology Tool Development: Serves as a core building block for creating advanced chemical probes to interrogate complex biological systems.

Basic Information

Product Name	3-(3-(3-(Trifluoromethyl)Diazirin-3-Yl)Phenyl)-2,3-Dihydroxypropionic Acid n-Hydroxysuccinimide Ester
CAS No.	143016-36-8
Molecular Formula	C15H12F3N3O6
Molecular Weight	387.27 g/mol
Synonyms	3-[3-[3-(Trifluoromethyl)-3H-diazirin-3-yl]phenyl]-2,3-dihydroxypropanoic acid 2,5-dioxopyrrolidin-1-yl ester; TFD Diazirine NHS Ester; 3-(3-(3-(Trifluoromethyl)diazirin-3-yl)phenyl)-2,3-dihydroxypropionic acid N-hydroxysuccinimide ester; Benzophenone-based Photoaffinity Labeling Reagent; Photo-leucine analog NHS ester; PAL Reagent; Trifluoromethylphenyl Diazirine NHS Ester; Succinimidyl 3-[3-[3-(trifluoromethyl)-3H-diazirin-3-yl]phenyl]-2,3-dihydroxypropanoate
EINECS	Contact for details

Quality Control

Every batch of our 3-(3-(3-(Trifluoromethyl)Diazirin-3-Yl)Phenyl)-2,3-Dihydroxypropionic Acid n-Hydroxysuccinimide Ester is synthesized and purified under controlled conditions to ensure the highest standards of purity and reactivity. We employ rigorous analytical protocols, including HPLC, NMR, and MS, to verify identity and potency. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) due to its hygroscopic and light-sensitive nature. For long-term storage, keep under an inert atmosphere (argon or nitrogen) to maximize stability. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.