Description

Suc-Ala-Leu-Pro-Phe-Amc is a fluorogenic peptide substrate specifically designed for the detection and quantification of chymotrypsin-like protease activity. This compound is a critical tool in biochemical research and pharmaceutical development, enabling precise enzymatic studies. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical industries for high-throughput screening and mechanistic investigations.

Application

• Protease Activity Assays: A standard fluorogenic substrate for measuring the activity of chymotrypsin, cathepsin G, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Enzyme Kinetics Studies: Essential for determining kinetic parameters (Km, Vmax, kcat) of proteolytic enzymes in purified systems.
• Biochemical Research: Employed in cell biology and immunology research to study protease involvement in signaling pathways and disease mechanisms.
• Diagnostic Development: Serves as a key component in developing in-vitro diagnostic tests for conditions involving abnormal protease activity.
• Quality Control in Biologics: Used to monitor and validate the activity and specificity of enzyme-based biopharmaceutical products.

Basic Information

Product Name	Suc-Ala-Leu-Pro-Phe-Amc
CAS No.	142997-31-7
Molecular Formula	C₃₄H₄₄N₆O₁₀
Molecular Weight	696.75 g/mol
Synonyms	N-Succinyl-Ala-Leu-Pro-Phe-7-amido-4-methylcoumarin; Suc-ALPF-AMC; Suc-Ala-Leu-Pro-Phe-AMC; N-Succinyl-Ala-Leu-Pro-Phe-AMC; Chymotrypsin Substrate I; Protease Substrate Suc-ALPF-AMC; 7-Amido-4-methylcoumarin derivative of Suc-Ala-Leu-Pro-Phe; Fluorogenic Chymotrypsin Substrate
EINECS	Contact for details

Quality Control

Our Suc-Ala-Leu-Pro-Phe-Amc is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, typically ≥95% (HPLC). Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and absence of critical impurities. We support research and production needs that may require compliance with cGMP or other relevant quality guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.