Description

Suc-Ala-Glu-Pro-Phe-Amc CAS NO 142997-30-6 is a fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound is of critical importance in biochemical research and pharmaceutical development for studying enzyme kinetics and inhibitor screening. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors.

Application

• Protease Activity Assays: A key fluorogenic substrate for continuous, real-time monitoring of protease enzymatic activity, particularly for chymotrypsin-like proteases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors for therapeutic development.
• Biochemical Research: Essential tool for kinetic studies, determining enzyme parameters (Km, Vmax), and investigating protease specificity and mechanism of action.
• Diagnostic Development: Employed in the development of fluorometric diagnostic tests for diseases where specific protease activity is a biomarker.
• Quality Control in Biologics: Used to monitor and validate the activity of protease enzymes used in manufacturing processes.
• Cell Biology Studies: Applied in assays to detect and localize protease activity within cellular and tissue samples.

Basic Information

Product Name	Suc-Ala-Glu-Pro-Phe-Amc
CAS No.	142997-30-6
Molecular Formula	C₃₃H₄₀N₆O₁₁
Molecular Weight	696.71 g/mol
Synonyms	Suc-AEPF-AMC; N-Succinyl-Ala-Glu-Pro-Phe-7-amido-4-methylcoumarin; Suc-Ala-Glu-Pro-Phe-AMC; Substrate for Chymotrypsin; Fluorogenic Protease Substrate IV; Suc-AEPF-AMC; N-Succinyl-Ala-Glu-Pro-Phe-AMC; 7-Amido-4-methylcoumarin derivative of Suc-AEPF
EINECS	Contact for details

Quality Control

Our Suc-Ala-Glu-Pro-Phe-Amc is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We ensure compliance with relevant standards for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Functional Activity	Conforms to reference in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.