Description

2',3'-o-(1-Oxy-2,2,6,6-Tetramethyl-4-Piperidylidene)Adenosine 5'-Triphosphate is a chemically modified nucleotide analog, specifically a spin-labeled ATP derivative. This compound matters as a critical research tool in biochemistry and biophysics, enabling the study of enzyme kinetics, protein-nucleic acid interactions, and conformational dynamics through techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. It is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic developers working on nucleotide-binding proteins, kinases, ATPases, and other energy-transducing systems.

Application

• EPR/Spin-Labeling Studies: As a site-directed spin label for probing the structure, dynamics, and conformational changes of ATP-binding proteins and enzymes.
• Enzyme Kinetics & Mechanism: To investigate the binding affinity, catalytic cycle, and inhibition mechanisms of kinases, ATPases, helicases, and polymerases.
• Structural Biology: Aiding in the determination of protein structures and complex formation using advanced magnetic resonance techniques.
• Drug Discovery Screening: Serving as a specialized substrate or probe in high-throughput assays to identify and characterize novel enzyme inhibitors or modulators.
• Diagnostic Assay Development: Potential use in developing sensitive diagnostic tests that monitor specific enzymatic activities linked to diseases.
• Signal Transduction Research: Studying ATP-dependent processes in cellular signaling pathways.

Basic Information

Product Name	2',3'-o-(1-Oxy-2,2,6,6-Tetramethyl-4-Piperidylidene)Adenosine 5'-Triphosphate
CAS No.	142723-07-7
Molecular Formula	C23H34N7O16P3
Molecular Weight	781.48 g/mol
Synonyms	2',3'-O-(1-Oxy-2,2,6,6-tetramethyl-4-piperidinylidene)adenosine-5'-triphosphate; 2',3'-O-(2,2,6,6-Tetramethyl-4-piperidylidene-1-oxyl)adenosine 5'-triphosphate; SL-ATP; Spin-Labeled ATP; TEMPO-ATP; Adenosine-5'-triphosphate, 2',3'-O-(1-oxy-2,2,6,6-tetramethyl-4-piperidinylidene)-; ATP spin label; Nitroxide-labeled ATP
EINECS	Contact for details

Quality Control

Our 2',3'-o-(1-Oxy-2,2,6,6-Tetramethyl-4-Piperidylidene)Adenosine 5'-Triphosphate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.