Description

3,3'-Dithiobispropionic Acid Bis-Sulfosuccinimidyl Ester Disodium Salt 90% CAS NO 142702-31-6 is a water-soluble, homobifunctional NHS ester crosslinker containing a cleavable disulfide bond. This compound is critically important for its ability to create reversible, reducible covalent linkages between primary amines, enabling the study of protein-protein interactions and the development of cleavable bioconjugates. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, proteomics, antibody-drug conjugate (ADC) development, and diagnostic assay formulation.

Application

• Bioconjugation and Crosslinking: For covalently linking proteins, peptides, or other amine-containing biomolecules with the option for disulfide bond reduction.
• Antibody-Drug Conjugate (ADC) Linker Research: Serves as a model or intermediate for constructing cleavable linkers that release payloads in reducing environments.
• Proteomics and Protein Interaction Studies: Used to capture and identify interacting protein partners via reversible crosslinking.
• Diagnostic Reagent Development: Immobilization of enzymes or antibodies onto solid supports with controlled release capabilities.
• Polymer and Biomaterial Modification: Introducing reactive, reducible sites into hydrogels or polymeric scaffolds for controlled degradation.
• Affinity Chromatography Ligand Attachment: Coupling ligands to chromatography resins for purifications that require elution under mild reducing conditions.

Basic Information

Product Name	3,3'-Dithiobispropionic Acid Bis-Sulfosuccinimidyl Ester Disodium Salt 90%
CAS No.	142702-31-6
Molecular Formula	C16H16N2Na2O14S4
Molecular Weight	634.53 g/mol
Synonyms	DTSSP (3,3'-Dithiobis(sulfosuccinimidyl propionate)); Bis(sulfosuccinimidyl) 3,3'-dithiodipropionate; Disodium 3,3'-dithiobis(propionyloxysulfosuccinimide); Sulfo-DTSSP; Water-soluble DTSSP; Bis-NHS-SS-diacid disodium salt; Homobifunctional sulfo-NHS ester crosslinker; 3,3'-Dithiodipropionic acid bis(N-hydroxysulfosuccinimide) ester disodium salt
EINECS	Contact for details

Quality Control

Our 3,3'-Dithiobispropionic Acid Bis-Sulfosuccinimidyl Ester Disodium Salt is manufactured under a quality management system. Each batch undergoes rigorous analytical testing to ensure identity, purity, and functional performance for bioconjugation applications. Certificates of Analysis (COA) with detailed specifications from HPLC, NMR, and MS are provided to guarantee batch-to-batch consistency and support your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90.0%
Water Content (KF)	≤ 5.0%
Solubility (1% in Water)	Clear, colorless solution
Functional Activity	Conforms (by amine coupling test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.