Description

Abz-Gly-Ala-Ala-Pro-Phe-3-Nitro-Tyr-Asp-Oh is a synthetic, sequence-specific peptide substrate designed for enzymatic research and assay development. This compound is of critical importance for studying protease activity, particularly for enzymes like chymotrypsin or related serine proteases, due to its strategically incorporated fluorogenic leaving group (Abz) and chromogenic nitro-tyrosine residue. It is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on enzyme kinetics, inhibitor screening, and biochemical pathway analysis.

Application

• Protease Activity Assays: Serving as a specific, sensitive substrate for quantifying the activity of chymotrypsin-like proteases in vitro.
• Enzyme Kinetics & Inhibitor Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Biochemical Research: A key tool for investigating protease specificity, catalytic mechanisms, and substrate recognition profiles in academic and industrial labs.
• Diagnostic Development: Potential use as a core component in developing clinical diagnostic assays that measure specific protease levels.
• Quality Control in Biomanufacturing: Employed to monitor protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Abz-Gly-Ala-Ala-Pro-Phe-3-Nitro-Tyr-Asp-Oh
CAS No.	142689-23-4
Molecular Formula	C₅₀H₅₈N₁₀O₁₇
Molecular Weight	1055.06 g/mol
Synonyms	Abz-GAAPF-3-NO₂-Y-D-OH; 2-Aminobenzoic Acid-Gly-Ala-Ala-Pro-Phe-3-Nitro-Tyr-Asp-OH; o-Aminobenzoyl-Gly-Ala-Ala-Pro-Phe-3-Nitro-Tyr-Asp-OH; Fluorogenic Substrate for Chymotrypsin; Chymotrypsin Substrate Abz-GAAPF-3NO₂Y-D; Protease Substrate 142689-23-4; Peptide Substrate for Protease Assay
EINECS	Contact for details

Quality Control

Our Abz-Gly-Ala-Ala-Pro-Phe-3-Nitro-Tyr-Asp-Oh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and MS for identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.