Description

Biotin Disulfide n-Hydroxy-Succinimide Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for creating stable, cleavable linkages between biomolecules, such as proteins and nucleic acids, facilitating targeted delivery and labeling studies. It is an essential tool for researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced life science research.

Application

• Bioconjugation and Crosslinking: Primary reagent for introducing cleavable disulfide bonds between biotinylated probes and target molecules like antibodies or peptides.
• Drug Delivery Systems: Used in the development of targeted therapeutics and antibody-drug conjugates (ADCs) where controlled release in reducing environments is required.
• Diagnostic Assay Development: Key component in constructing sensitive ELISA, lateral flow, and other immunoassay platforms requiring stable, reversible immobilization.
• Affinity Chromatography: For the reversible immobilization of ligands on solid supports to create purification resins for proteins and other biomolecules.
• Cell Surface Labeling: Enables the specific tagging and subsequent isolation of cell surface receptors and other membrane proteins.
• Proteomics and Protein Interaction Studies: Facilitates the capture and analysis of protein complexes through cleavable biotin-streptavidin interactions.

Basic Information

Product Name	Biotin Disulfide n-Hydroxy-Succinimide Ester
CAS No.	142439-92-7
Molecular Formula	C19H28N4O6S2
Molecular Weight	472.58 g/mol
Synonyms	Biotin-SS-NHS; Biotin Disulfide NHS Ester; NHS-SS-Biotin; (+)-Biotin N-hydroxysuccinimide ester disulfide; 1-Biotinamido-4-[4'-(N-hydroxysuccinimidyloxycarbonyl)butyl]disulfide; N-Succinimidyl 3-[(2-biotinamido)ethyldithio]propionate; SBED; Succinimidyl 3-((2-(biotinamido)ethyl)dithio)propanoate
EINECS	Contact for details

Quality Control

Our Biotin Disulfide n-Hydroxy-Succinimide Ester is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the stringent requirements for research and bioprocessing. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF; slightly soluble in acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.