Description

5'-O-(4,4'-Dimethoxytrityl)-5-Fluorodeoxyuridine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is a critical phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is essential for the precise and efficient incorporation of 5-fluorodeoxyuridine, a key pyrimidine analog, into synthetic DNA strands. It is primarily required by biotechnology, pharmaceutical, and diagnostic research organizations engaged in the development of antisense oligonucleotides, primers, probes, and therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: Primary building block for the automated solid-phase synthesis of DNA strands containing 5-fluorodeoxyuridine.
• Antisense & siRNA Development: Synthesis of modified oligonucleotides for gene silencing and therapeutic research applications.
• Diagnostic Probe Manufacturing: Production of fluorescently labeled or otherwise tagged probes for PCR, FISH, and microarray technologies.
• Structural Biology Studies: Incorporation of fluorinated nucleotides to study nucleic acid structure, stability, and protein interactions.
• Pharmaceutical R&D: Key raw material in the research and development of novel oligonucleotide-based therapeutics.
• Primer Synthesis: Manufacture of specialized primers for sequencing and amplification techniques requiring modified bases.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-5-Fluorodeoxyuridine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite
CAS No.	142246-63-7
Molecular Formula	C41H48FN4O8P
Molecular Weight	774.82 g/mol
Synonyms	5-Fluoro-2'-deoxyuridine 5'-O-(4,4'-dimethoxytrityl)-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite; 5-FdU Phosphoramidite; 5-Fluorodeoxyuridine Phosphoramidite; 5-Fluoro-dU Phosphoramidite; DMT-5-Fluorodeoxyuridine-CE Phosphoramidite; 5-F-dU-CE Phosphoramidite; 5-Fluorouridine Deoxy Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-5-Fluorodeoxyuridine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC, 31P NMR, and 1H NMR, to confirm identity, purity, and the absence of critical impurities. Certificates of Analysis (COA) with detailed specifications are provided to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will lead to decomposition and reduced coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.