Description

2,4-Dinitrophenyl-Valyl-2-Aminoisobutyryl-Glycyl-Leucyl-4-Nitroanilide is a specialized synthetic peptide derivative designed for advanced biochemical research and development. This compound serves as a critical chromogenic substrate for the precise detection and kinetic analysis of proteolytic enzyme activity, particularly in high-throughput screening environments. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable and sensitive reagents for enzyme characterization and inhibitor studies.

Application

• Protease/Peptidase Substrate: Primary application as a chromogenic substrate for the kinetic study and quantification of various proteolytic enzymes, including elastase, cathepsin, and other serine or cysteine proteases.
• High-Throughput Screening (HTS): Used in automated assay systems within pharmaceutical R&D for the discovery and evaluation of novel enzyme inhibitors as potential drug candidates.
• Biochemical Research: A key reagent in fundamental enzymology research to investigate enzyme mechanism, specificity, and catalytic efficiency.
• Diagnostic Kit Development: Employed in the development of in-vitro diagnostic (IVD) assays for clinical analysis of enzyme levels related to specific physiological conditions or diseases.
• Quality Control in Biomanufacturing: Utilized to monitor and validate the activity and purity of enzyme preparations used in biopharmaceutical production processes.

Basic Information

Product Name	2,4-Dinitrophenyl-Valyl-2-Aminoisobutyryl-Glycyl-Leucyl-4-Nitroanilide
CAS No.	141949-33-9
Molecular Formula	C31H41N9O10
Molecular Weight	699.72 g/mol
Synonyms	DNP-Val-Aib-Gly-Leu-pNA; 2,4-DNP-Val-Aib-Gly-Leu-4-NA; Val-Aib-Gly-Leu-p-Nitroanilide DNP derivative; Chromogenic elastase substrate; Protease substrate 141949-33-9; N-2,4-Dinitrophenyl-L-valyl-2-aminoisobutyryl-glycyl-L-leucyl-4-nitroanilide; L-Leucine, N-[N-[N-[N2-(2,4-dinitrophenyl)-L-valyl]-2-aminoisobutyryl]glycyl]-, 4-nitroanilide
EINECS	Contact for details

Quality Control

Every batch of 2,4-Dinitrophenyl-Valyl-2-Aminoisobutyryl-Glycyl-Leucyl-4-Nitroanilide is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance consistency suitable for research applications. Our quality assurance protocol includes advanced chromatographic and spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, moisture, or light may affect stability and performance. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.