Description

Benzyloxycarbonyl-L-Valyl-L-Lysyl-L-Methionine 4-Methylcoumaryl-7-Amide Hydrochloride is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of specific protease activity. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic kit manufacturing for studying enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: As a fluorogenic substrate for specific proteolytic enzymes, enabling real-time, high-throughput screening.
• Biochemical Research: Used in academic and industrial labs to study enzyme mechanisms, kinetics, and inhibition profiles.
• Pharmaceutical R&D: Essential for drug discovery programs targeting proteases, facilitating the identification and validation of novel therapeutic candidates.
• Diagnostic Development: Incorporated into diagnostic kits and reagents for the detection of specific enzyme markers related to various physiological or pathological conditions.
• Quality Control in Biologics: Employed to monitor and ensure the consistency and activity of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	Benzyloxycarbonyl-L-Valyl-L-Lysyl-L-Methionine 4-Methylcoumaryl-7-Amide Hydrochloride
CAS No.	141223-71-4
Molecular Formula	C37H51N7O8S • HCl
Molecular Weight	806.37 g/mol (Free base: 769.92 g/mol)
Synonyms	Z-Val-Lys-Met-AMC • HCl; Z-Val-Lys-Met-AMC hydrochloride; Cbz-Val-Lys-Met-AMC • HCl; Benzyloxycarbonyl-Val-Lys-Methionine 7-Amido-4-methylcoumarin Hydrochloride; Fluorogenic Substrate for Proteases; 4-Methylcoumaryl-7-amide Substrate; Protease Substrate V; CAS 141223-71-4
EINECS	Contact for details

Quality Control

Every batch of our Benzyloxycarbonyl-L-Valyl-L-Lysyl-L-Methionine 4-Methylcoumaryl-7-Amide Hydrochloride is manufactured and tested under strict quality management systems. We ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Comprehensive testing includes identity confirmation, purity assay, and impurity profiling. A Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.