Description

3'-Azido-3'-Deoxythymidine 5'-(Beta,γ-Imido)Triphosphate is a chemically modified nucleotide analog, specifically a non-hydrolyzable ATP analog where the β-gamma oxygen bridge is replaced by an imido group. This compound is critical for biochemical research as it acts as a potent inhibitor of ATP-dependent enzymes, allowing for the study of enzyme kinetics and mechanisms without substrate turnover. It is primarily utilized by researchers in molecular biology, enzymology, and pharmaceutical development for investigating nucleic acid metabolism and as a tool compound in high-throughput screening assays.

Application

• Enzyme Inhibition Studies: Used as a non-hydrolyzable ATP analog to inhibit DNA polymerases, reverse transcriptases, and other ATP-dependent enzymes to study reaction mechanisms.
• Biochemical Assay Development: Serves as a critical reagent in the development of assays for screening potential antiviral or anticancer drug candidates that target nucleotide-binding sites.
• Nucleic Acid Research: Employed in studies of DNA replication and repair processes to understand the role of ATP hydrolysis in these complex pathways.
• Structural Biology: Used in X-ray crystallography and NMR studies to obtain enzyme-inhibitor complex structures, providing insights into active site architecture.
• Kinase Research: Acts as a substrate analog in studies of protein kinases and other phosphotransferases to elucidate phosphorylation mechanisms.

Basic Information

Product Name	3'-Azido-3'-Deoxythymidine 5'-(Beta,γ-Imido)Triphosphate
CAS No.	141171-21-3
Molecular Formula	C11H16N6O12P3
Molecular Weight	517.20 g/mol
Synonyms	AZT-Imido-ATP; 3'-Azido-3'-deoxythymidine 5'-(β,γ-imido)triphosphate; Adenylyl imidodiphosphate AZT conjugate; AZT-PNP; 5'-O-[(β,γ-Imido)triphosphoryl]-3'-azido-3'-deoxythymidine; Non-hydrolyzable AZT triphosphate analog; β,γ-Imido-ATP analog of AZT
EINECS	Contact for details

Quality Control

Our 3'-Azido-3'-Deoxythymidine 5'-(Beta,γ-Imido)Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, essential for sensitive research applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, the container should be allowed to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.