Description

Thymidine 5'-(Alpha,β-Imido)Triphosphate CAS NO 141171-14-4 is a chemically modified, non-hydrolyzable analog of deoxythymidine triphosphate (dTTP). This compound is critical for research applications where a stable, ATP-competitive inhibitor or a non-reactive phosphate donor is required. It is primarily utilized in biochemical and pharmacological studies, including enzyme kinetics, nucleic acid research, and as a tool compound in signal transduction pathway analysis.

Application

• Enzyme Inhibition Studies: Used as a potent and specific inhibitor for ATP-dependent enzymes, including various kinases and helicases.
• Nucleic Acid Biochemistry: Serves as a non-hydrolyzable substrate analog in studies of DNA/RNA polymerases and other nucleic acid-metabolizing enzymes.
• Signal Transduction Research: Employed to investigate ATP-mediated signaling pathways without the complication of phosphate transfer.
• Biochemical Assay Development: Acts as a key component in high-throughput screening (HTS) assays for drug discovery targeting nucleotide-binding sites.
• Structural Biology: Used in X-ray crystallography and NMR studies to capture enzyme-substrate complex structures.
• Control Experiments: Provides a negative control in experiments requiring an inactive nucleotide triphosphate analog.

Basic Information

Product Name	Thymidine 5'-(Alpha,β-Imido)Triphosphate
CAS No.	141171-14-4
Molecular Formula	C10H17N4O13P3
Molecular Weight	502.18 g/mol
Synonyms	Thymidine 5'-(α,β-Imido)triphosphate; dTMP-PNP; Adenosine 5'-(α,β-Imido)triphosphate (Thymidine analog); 2'-Deoxythymidine 5'-(α,β-imido)triphosphate; Non-hydrolyzable dTTP analog; Imido-dTTP; dTpNHp; TNP
EINECS	Contact for details

Quality Control

Our Thymidine 5'-(Alpha,β-Imido)Triphosphate is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for life science research. A detailed Certificate of Analysis (COA) is provided with each shipment, guaranteeing traceability and consistent performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.