Description

4-Nitrophenyl 2-Acetamido-6-o-(2-Acetamido-2-Deoxy-B-D-Glucopyranosyl) -3-o-(B-D-Galactopyranosyl)-2-Deoxy-A- D-Galactopyranoside is a specialized, high-purity synthetic oligosaccharide derivative designed for advanced biochemical research and diagnostic applications. This compound serves as a critical chromogenic substrate for the detection and kinetic study of specific glycosidase enzymes. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical R&D laboratories focused on glycobiology, enzymology, and the development of novel assays.

Application

• Enzyme Substrate for Glycosidases: Acts as a specific chromogenic substrate for α-N-acetylgalactosaminidase and related enzymes, producing a measurable color change upon cleavage.
• Diagnostic Reagent Manufacturing: A key raw material in the production of diagnostic kits for genetic disorders like Schindler/Kanzaki disease, which involve deficiencies in specific lysosomal enzymes.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibition assays within academic and industrial glycobiology research.
• Quality Control in Biopharma: Employed in QC laboratories to test the activity of enzyme-based therapeutics or recombinant enzymes.
• High-Throughput Screening (HTS): Suitable for use in HTS platforms to identify potential therapeutic modulators of glycosidase activity.

Basic Information

Product Name	4-Nitrophenyl 2-Acetamido-6-o-(2-Acetamido-2-Deoxy-B-D-Glucopyranosyl) -3-o-(B-D-Galactopyranosyl)-2-Deoxy-A- D-Galactopyranoside
CAS No.	139459-55-5
Molecular Formula	C34H49N3O21
Molecular Weight	835.76 g/mol
Synonyms	p-Nitrophenyl-α-D-GalNAc-(1→3)-[α-D-GlcNAc-(1→6)]-β-D-GalNAc; 4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-6-O-[2-acetamido-2-deoxy-β-D-glucopyranosyl]-α-D-galactopyranoside; α-D-GalNAc-(1→3)-[α-D-GlcNAc-(1→6)]-D-GalNAc-pNP; PNP-α-D-GalNAc-(1→3)-[α-D-GlcNAc-(1→6)]-D-GalNAc; Chromogenic substrate for α-N-acetylgalactosaminidase; Schindler disease diagnostic substrate.
EINECS	Contact for details

Quality Control

Every batch of this high-value biochemical is produced and analyzed under strict quality management protocols. We ensure compliance with exacting standards suitable for research and in-vitro diagnostic (IVD) use. Comprehensive testing includes identity confirmation, purity assay, and specific activity verification. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity (typically >95% by HPLC), spectral data, and performance in standard enzymatic assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store under desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Enzymatic Activity	Suitable for use as substrate (performance data on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.