Description

Serine-7-Amino-4-Methylcoumarin Carbamate is a specialized fluorogenic substrate and biochemical reagent designed for high-sensitivity detection. This compound matters for its role as a key component in enzymatic assays, where it serves as a cleavable substrate that releases a highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety. It is primarily needed by researchers and manufacturers in the fields of protease and peptidase research, high-throughput screening (HTS), and diagnostic kit development.

Application

• Protease & Peptidase Activity Assays: Used as a specific fluorogenic substrate to measure the enzymatic activity of serine proteases and other hydrolytic enzymes.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize potential enzyme inhibitors or activators.
• Biochemical Research: Serves as a critical tool for studying enzyme kinetics, mechanism of action, and substrate specificity in vitro.
• Diagnostic Development: Incorporated into fluorometric diagnostic kits and assays for clinical and research applications requiring high sensitivity.
• Cell-Based Assays: Utilized in live-cell imaging and intracellular protease activity monitoring due to the cell-permeant nature of certain derivatives.

Basic Information

Product Name	Serine-7-Amino-4-Methylcoumarin Carbamate
CAS No.	139262-16-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Serine-AMC Carbamate; L-Serine-7-amido-4-methylcoumarin; AMC-Serine Carbamate; Fluorogenic Serine Protease Substrate; 7-Amino-4-methylcoumarin conjugate of Serine; Protease Substrate for Serine Hydrolases; (S)-2-(((7-Amino-4-methyl-2-oxo-2H-chromen-3-yl)oxy)carbonylamino)-3-hydroxypropanoic acid
EINECS	Contact for details

Quality Control

Our Serine-7-Amino-4-Methylcoumarin Carbamate is produced under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. Keep the container in a dry, cool, and well-ventilated area.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.