Description

5-Dodecanoylaminofluorescein di-β-D-galactopyranoside is a specialized fluorogenic substrate designed for the sensitive detection of β-galactosidase activity. This compound matters for researchers and quality control professionals who require precise, high-throughput enzymatic assays. It is primarily needed in the biotechnology, pharmaceutical, and molecular biology sectors for applications such as reporter gene assays, cell viability studies, and enzyme kinetics.

Application

• Reporter Gene Assays: A critical substrate for detecting β-galactosidase expression in molecular biology and genetic engineering.
• Cell-Based Screening: Used in high-throughput screening (HTS) platforms to identify modulators of gene expression or enzyme activity.
• Enzyme Kinetics & Characterization: Employed for the detailed study of β-galactosidase kinetics, inhibition, and specificity.
• Microbial Detection: Applied in microbiology for the identification of bacterial species (e.g., E. coli) expressing β-galactosidase.
• Apoptosis & Cell Viability Assays: Utilized in assays measuring cell health, where β-galactosidase activity serves as a marker.
• Diagnostic Development: Serves as a key component in developing diagnostic kits and biosensors for enzymatic activity.

Basic Information

Product Name	5-Dodecanoylaminofluorescein di-β-D-galactopyranoside
CAS No.	138777-25-0
Molecular Formula	C46H56NO18
Molecular Weight	886.93 g/mol
Synonyms	5-Dodecanoylaminofluorescein di-β-D-galactoside; FDG (C12); Fluorescein di-β-D-galactopyranoside (C12 derivative); 5-(Dodecanoylamino)fluorescein di-β-D-galactopyranoside; β-Galactosidase Substrate (Fluorogenic, C12); C12-FDG; Long-chain alkyl derivative of FDG; 5-(N-Dodecanoyl)aminofluorescein di-β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our 5-Dodecanoylaminofluorescein di-β-D-galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the product meets the stringent requirements for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or elevated temperatures will compromise stability and performance. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Functional in standard β-galactosidase assay
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.