Description

Anthranilyl-HIV Protease Substrate III is a specialized fluorogenic peptide substrate designed for the sensitive detection and kinetic analysis of HIV-1 protease activity. This compound is critical for biochemical research and pharmaceutical development focused on antiviral therapies. It is primarily utilized by researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic sectors for high-throughput screening and enzymatic assay development.

Application

• HIV Protease Activity Assays: As a fluorogenic substrate for continuous, real-time monitoring of HIV-1 protease enzyme kinetics in vitro.
• High-Throughput Screening (HTS): For the identification and evaluation of potential HIV protease inhibitor candidates in drug discovery programs.
• Biochemical Research: Used in mechanistic studies to understand substrate specificity and catalytic efficiency of retroviral proteases.
• Diagnostic Development: Serves as a key component in developing sensitive fluorometric assays for protease detection in research settings.
• Enzyme Characterization: For determining kinetic parameters (Km, Vmax, kcat) of HIV protease and related mutant enzymes.
• Quality Control Testing: Employed in the QC of recombinant HIV protease production and batch consistency verification.

Basic Information

Product Name	Anthranilyl-HIV Protease Substrate III
CAS No.	138668-80-1
Molecular Formula	C46H62N10O11
Molecular Weight	907.05 g/mol
Synonyms	Anthranilyl-HIV PR Substrate III; MCA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(Dnp)-Arg-NH2; 7-Methoxycoumarin-4-acetyl-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(Dnp)-Arg-amide; Fluorogenic HIV-1 Protease Substrate; HIV-1 Protease Fluorogenic Peptide Substrate; MCA-based HIV Protease Substrate; RETRO-THF HIV Protease Substrate (Analog)
EINECS	Contact for details

Quality Control

Every batch of Anthranilyl-HIV Protease Substrate III is manufactured and tested under strict quality management protocols to ensure high purity and consistent performance. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for research and assay development. Certificates of Analysis (COA) with detailed chromatographic data are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (Karl Fischer)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Cleaved by HIV-1 protease to yield fluorescent signal

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.