Description

tert-Butyloxycarbonyl-1-Alanine 4-Methylumbelliferyl Ester is a specialized fluorogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the sensitive detection and quantification of protease activity, particularly for enzymes like aminopeptidases. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, enzyme characterization, and assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous monitoring of aminopeptidase and related protease enzyme activity.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential enzyme inhibitors or activators.
• Biochemical Research: A key tool for studying enzyme kinetics, specificity, and mechanism of action in academic and industrial laboratories.
• Diagnostic Kit Development: Incorporated into diagnostic assays for detecting specific enzymatic markers associated with diseases.
• Quality Control in Biomanufacturing: Employed to monitor the presence and activity of specific proteases during biopharmaceutical production processes.

Basic Information

Product Name	tert-Butyloxycarbonyl-1-Alanine 4-Methylumbelliferyl Ester
CAS No.	138474-38-1
Molecular Formula	C18H21NO6
Molecular Weight	347.36 g/mol
Synonyms	Boc-Ala-4-methylumbelliferyl ester; Boc-L-Alanine 4-methyl-7-umbelliferyl ester; N-(tert-Butoxycarbonyl)-L-alanine 4-methylumbelliferyl ester; 4-Methylumbelliferyl N-Boc-L-alaninate; L-Alanine, N-[(1,1-dimethylethoxy)carbonyl]-, 4-methyl-2-oxo-2H-1-benzopyran-7-yl ester; Boc-Ala-MCA (related class); Fluorogenic aminopeptidase substrate
EINECS	Contact for details

Quality Control

Our tert-Butyloxycarbonyl-1-Alanine 4-Methylumbelliferyl Ester is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.