Description

6-Chloro-3-Indolyl-β-D-Galactopyranoside is a chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its ability to produce an insoluble blue indigo dye upon enzymatic hydrolysis, serving as a critical visual indicator for gene expression and enzyme activity. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic kit production industries. It is a key reagent for techniques such as blue-white screening in cloning and histochemical staining.

Application

• Molecular Biology & Cloning: As a chromogenic substrate for β-galactosidase in blue-white screening of recombinant bacterial colonies (e.g., pUC, M13 vectors).
• Diagnostic Assays: Used in enzymatic immunoassays (EIAs) and lateral flow tests to detect specific antigens or antibodies via color development.
• Histochemistry & Cell Biology: For staining tissues and cells to localize β-galactosidase activity, commonly used in reporter gene studies and senescence assays.
• Enzyme Activity Analysis: Quantitative and qualitative measurement of β-galactosidase enzyme kinetics and inhibition studies in research.
• Microbiology: As a component in selective culture media for the identification of microorganisms based on galactosidase activity.
• Biochemical Research: A tool for studying gene regulation, promoter activity, and signal transduction pathways in various model organisms.

Basic Information

Product Name	6-Chloro-3-Indolyl-β-D-Galactopyranoside
CAS No.	138182-21-5
Molecular Formula	C14H16ClNO6
Molecular Weight	329.73 g/mol
Synonyms	6-Chloro-3-indolyl β-D-galactopyranoside; 6-Chloro-3-indolyl-β-D-galactoside; 6-Cl-β-Gal; X-Gal (6-Chloro derivative); 6-Chloroindolyl-β-D-galactoside; 6-Chloro-1H-indol-3-yl β-D-galactopyranoside; Magenta-Gal; BCIG (6-Chloro-3-indolyl-β-D-galactopyranoside)
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indolyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request. We support compliance with relevant quality standards for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, moisture, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Melting Point	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.