Description

4-Methylumbelliferyl3,4,6-Tri-o-Acetyl-2-Deoxy-2-Trifluoroacetamido-B-D-Glucopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of specific glycosidase enzyme activity. This compound matters because it provides a critical tool for biochemical research, enabling high-throughput screening and precise enzymatic assays in diagnostic and pharmaceutical development. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical R&D, and diagnostic kit production sectors.

Application

• Enzymatic Assay Substrate: A key fluorogenic substrate for detecting and quantifying the activity of β-N-acetylhexosaminidase and related enzymes.
• Biochemical Research: Used in fundamental research to study glycosidase function, enzyme kinetics, and inhibition mechanisms.
• Diagnostic Development: Employed in the development of diagnostic assays for genetic disorders like Tay-Sachs and Sandhoff diseases, where enzyme deficiency is a marker.
• High-Throughput Screening (HTS): Utilized in pharmaceutical discovery for screening compound libraries for potential glycosidase inhibitors or activators.
• Cell Biology Studies: Applied in cell-based assays to visualize and measure enzymatic activity within live or lysed cells.
• Quality Control in Biomanufacturing: Serves as a reagent for monitoring the activity of enzyme-based biopharmaceutical products during production.

Basic Information

Product Name	4-Methylumbelliferyl3,4,6-Tri-o-Acetyl-2-Deoxy-2-Trifluoroacetamido-B-D-Glucopyranoside
CAS No.	137686-93-2
Molecular Formula	C₂₄H₂₆F₃NO₁₂
Molecular Weight	577.46 g/mol
Synonyms	4-MU-3,4,6-Tri-O-acetyl-2-deoxy-2-trifluoroacetamido-β-D-glucopyranoside; 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide derivative; 4-MU-β-GlcNAc-3,4,6-triacetate; Fluorogenic N-acetyl-β-D-glucosaminide substrate; 2-Deoxy-2-(trifluoroacetamido)-β-D-glucopyranoside 4-methylumbelliferyl ester 3,4,6-triacetate; 4-MU-GlcNAc-3,4,6-TriAc; Hexosaminidase fluorogenic substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl3,4,6-Tri-o-Acetyl-2-Deoxy-2-Trifluoroacetamido-B-D-Glucopyranoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol
Fluorogenic Activity	Functional in standard hexosaminidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.