Description

4-Methylumbelliferyl3,4,6-Tri-o-Acetyl-2-Deoxy-2-Trifluoroacetamido-A-D-Glucopyranoside is a specialized fluorogenic glycosidase substrate, a critical reagent for enzyme activity assays and diagnostic research. Its core value lies in its ability to generate a highly fluorescent 4-methylumbelliferone (4-MU) product upon enzymatic cleavage, enabling sensitive and quantitative detection. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors.

Application

• Glycosidase Enzyme Assays: A key substrate for measuring the activity of specific glucosidases and other glycosyl hydrolases in research and high-throughput screening.
• Diagnostic Kit Development: Used as a core component in the formulation of diagnostic kits for inherited metabolic disorders, such as lysosomal storage diseases.
• Biochemical Research: Employed in mechanistic studies of enzyme kinetics, inhibition, and substrate specificity for carbohydrate-active enzymes.
• Pharmaceutical Quality Control: Serves as a reference standard or assay reagent in the QC testing of enzyme-based therapeutics.
• Cell-Based Screening: Utilized in cell culture assays to monitor glycosidase activity as a reporter for cellular processes or drug effects.
• Microbiology: Applied in assays to detect and characterize glycosidase activity in microbial cultures for identification purposes.

Basic Information

Product Name	4-Methylumbelliferyl3,4,6-Tri-o-Acetyl-2-Deoxy-2-Trifluoroacetamido-A-D-Glucopyranoside
CAS No.	137686-92-1
Molecular Formula	C24H24F3NO12
Molecular Weight	575.44 g/mol
Synonyms	4-MU-3,4,6-Tri-O-acetyl-2-deoxy-2-trifluoroacetamido-α-D-glucopyranoside; 4-Methylumbelliferyl 2-Deoxy-2-trifluoroacetamido-α-D-glucopyranoside triacetate; 4-MU-GlcNAc-3,4,6-Triacetate; Fluorogenic N-acetyl-β-D-glucosaminidase substrate (acetylated); α-4-Methylumbelliferyl N-acetyl-α-D-glucosaminide triacetate; 2-Deoxy-2-(trifluoroacetamido)-α-D-glucopyranosyl 4-methylumbelliferone 3,4,6-triacetate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl3,4,6-Tri-o-Acetyl-2-Deoxy-2-Trifluoroacetamido-A-D-Glucopyranoside is produced under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, identity confirmation (IR, NMR), and specific optical rotation measurement. Certificates of Analysis (COA) with detailed chromatograms are provided to guarantee the product meets the stringent specifications required for enzymatic and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.