Description

Digoxigenin-11-2',3'-Dideoxyuridine 5'-Triphosphate is a chemically modified nucleotide triphosphate where a digoxigenin hapten is conjugated to a chain-terminating dideoxyuridine nucleotide. This compound is a critical reagent for the non-radioactive labeling and detection of nucleic acids in molecular biology and diagnostic applications. It is primarily utilized by researchers and manufacturers in the fields of molecular diagnostics, genomics research, and in situ hybridization to create highly sensitive and specific probes.

Application

• Non-Radioactive DNA/RNA Labeling: Used as a substrate for DNA polymerases (e.g., terminal transferase) to incorporate digoxigenin tags into nucleic acid probes for blotting techniques (Southern, Northern, dot/slot blots).
• In Situ Hybridization (ISH): Essential for generating labeled probes to detect specific DNA or RNA sequences within cells and tissue sections with high spatial resolution.
• Microarray Fabrication: Employed in the synthesis of labeled nucleotides for spotting onto arrays or for target labeling in gene expression profiling.
• Diagnostic Assay Development: A key component in the development of clinical diagnostic kits for pathogen detection, genetic disorders, and cancer biomarkers.
• Chain Termination Sequencing: Acts as a labeled terminator in older sequencing methodologies, though largely superseded by newer technologies.
• Cytogenetics: Used in fluorescence in situ hybridization (FISH) and chromosome painting techniques when combined with anti-digoxigenin antibodies conjugated to fluorophores.

Basic Information

Product Name	Digoxigenin-11-2',3'-Dideoxyuridine 5'-Triphosphate
CAS No.	137067-07-3
Molecular Formula	C₃₈H₅₀N₄O₂₃P₃
Molecular Weight	1012.78 g/mol
Synonyms	Digoxigenin-11-ddUTP; DIG-11-ddUTP; 2',3'-Dideoxyuridine-5'-triphosphate, 11-(3,12-dihydroxy-24-oxocholan-24-yl)-; Digoxigenin-11-2'-deoxyuridine-5'-triphosphate (common misnomer); 11-Digoxigenin-ddUTP; DIG-ddUTP; Dideoxyuridine triphosphate-digoxigenin conjugate; Anti-Digoxigenin labeling nucleotide
EINECS	Contact for details

Quality Control

Our Digoxigenin-11-ddUTP is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, spectrophotometric analysis for concentration and labeling efficiency, and functional testing in enzymatic incorporation assays. A detailed Certificate of Analysis (COA) providing purity, identity, and performance data is supplied with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 3.0%
Enzymatic Incorporation Efficiency	≥ 90% (vs. control nucleotide)
Solubility	Soluble in aqueous buffer or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.