Description

5-Amino-(2,2,6,6-Tetramethylpiperidine-n-Oxyl)Uridine 5'-Triphosphate is a specialized, spin-labeled nucleotide analog that serves as a critical molecular probe in advanced biochemical research. Its core value lies in enabling the study of nucleic acid dynamics, enzyme mechanisms, and structural biology through techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. This compound is essential for researchers and developers in biotechnology, pharmaceutical discovery, and academic institutions who require precise tools for investigating biomolecular interactions and conformational changes.

Application

• EPR/ESR Spectroscopy: As a site-directed spin label (SDSL) for studying the structure, dynamics, and conformational changes of RNA and DNA.
• Enzyme Kinetics & Mechanism Studies: Probing the active sites and catalytic mechanisms of polymerases, kinases, and other nucleotide-processing enzymes.
• Nucleic Acid-Protein Interaction Mapping: Investigating binding interfaces and distance constraints in ribonucleoprotein complexes and transcription factor assemblies.
• Structural Biology: Aiding in the determination of macromolecular structures and dynamics through integration with other biophysical methods like NMR and cryo-EM.
• Biophysical Probe Development: Serving as a precursor for synthesizing custom-labeled oligonucleotides for advanced diagnostic and sensor applications.
• High-Throughput Screening (HTS): Enabling label-free or alternative detection methods in assays targeting nucleic acid metabolism.

Basic Information

Product Name	5-Amino-(2,2,6,6-Tetramethylpiperidine-n-Oxyl)Uridine 5'-Triphosphate
CAS No.	136134-11-7
Molecular Formula	C18H30N5O16P3
Molecular Weight	665.37 g/mol
Synonyms	5-Amino-TEMPO-Uridine 5'-Triphosphate; 5-ATU-TP; 5-Amino-2,2,6,6-tetramethylpiperidine-1-oxyl Uridine Triphosphate; Spin-labeled UTP; TEMPO-Amino-UTP; 5-(Aminomethyl)-UTP TEMPO derivative; 5-ATUTP; EPR-active UTP analog
EINECS	Contact for details

Quality Control

Every batch of our 5-Amino-(2,2,6,6-Tetramethylpiperidine-n-Oxyl)Uridine 5'-Triphosphate is produced and analyzed under stringent quality protocols to ensure the high purity and consistency required for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as purity (HPLC), spin label integrity (EPR), and absence of contaminating nucleases. Our quality system is designed to meet the exacting standards of research and development laboratories worldwide.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere (argon or nitrogen) and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference standard
Identification (EPR)	Active spin label present
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.