Description

7-Deaza-2'-Deoxyinosine Triphosphate is a chemically modified nucleotide analog, specifically a triphosphate derivative of 7-deaza-2'-deoxyinosine. This compound is critical for advanced molecular biology research, particularly in applications requiring reduced background fluorescence and enhanced signal detection. It is primarily utilized by researchers and manufacturers in the fields of next-generation sequencing (NGS), polymerase chain reaction (PCR), and nucleic acid diagnostics.

Application

• Next-Generation Sequencing (NGS): Used as a modified dNTP to reduce sequencing artifacts and improve read accuracy, especially in pyrosequencing and other sequencing-by-synthesis platforms.
• Polymerase Chain Reaction (PCR): Employed in specialized PCR applications to study polymerase fidelity and to incorporate modified bases into DNA for functional studies.
• DNA Labeling and Detection: Serves as a substrate for enzymatic incorporation of modified nucleotides for fluorescent or chemiluminescent labeling of nucleic acid probes.
• Enzymology Studies: Used as a tool to investigate the substrate specificity and kinetics of DNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Antiviral Research: Investigated for its potential as a chain terminator or inhibitor in the study of viral replication mechanisms.
• Diagnostic Assay Development: Incorporated into molecular diagnostic kits to enhance specificity and sensitivity in pathogen detection.

Basic Information

Product Name	7-Deaza-2'-Deoxyinosine Triphosphate
CAS No.	136120-28-0
Molecular Formula	C11H17N4O13P3
Molecular Weight	530.18 g/mol
Synonyms	7-Deaza-dITP; 7-Deaza-2'-deoxyinosine-5'-triphosphate; 2'-Deoxy-7-deazainosine triphosphate; 7-C-aza-2'-deoxyinosine triphosphate; d(7-deazaI)TP; 7-Deaza-2'-deoxyinosine 5'-(tetrahydrogen triphosphate); 7-Deaza-dInoTP; 7-Deaza-2'-deoxyinosine-5'-triphosphoric acid
EINECS	Contact for details

Quality Control

Our 7-Deaza-2'-Deoxyinosine Triphosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry for identity verification. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all critical quality attributes to ensure the material meets the stringent requirements for life science research and in-vitro diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated at -20°C. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
pH (1% Solution)	6.0 - 8.0
Endotoxin	< 0.1 EU/µmol
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.