Description

2,3-Bis(4-Chlorophenyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt widely used as a vital reagent in biochemical and analytical chemistry. Its core value lies in its role as a redox indicator and electron acceptor, particularly in assays for cell viability and dehydrogenase enzyme activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors who require reliable and sensitive detection methods.

Application

• Cell Viability and Cytotoxicity Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in living cells.
• Dehydrogenase Enzyme Detection: Serves as an electron acceptor in enzymatic reactions for detecting various dehydrogenase enzymes in biochemical research.
• Microbiological Testing: Employed in microbial viability tests and for monitoring bacterial growth and metabolic activity.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to evaluate the effects of drug candidates on cell metabolism.
• Toxicology Studies: Applied in in vitro toxicology to assess the impact of chemicals and environmental toxins on cellular health.
• Research & Development: A critical tool in academic and industrial R&D for studying cellular proliferation, apoptosis, and metabolic pathways.

Basic Information

Product Name	2,3-Bis(4-Chlorophenyl)-5-Phenyltetrazolium Chloride
CAS No.	135788-08-8
Molecular Formula	C20H13Cl3N4
Molecular Weight	415.70 g/mol
Synonyms	2,3-Bis(p-chlorophenyl)-5-phenyl-2H-tetrazolium chloride; 2,3-Di(4-chlorophenyl)-5-phenyltetrazolium chloride; Tetrazolium Chloride, 2,3-Bis(4-chlorophenyl)-5-phenyl-; MTT Tetrazolium Salt Analog; TTC Derivative; NSC 406050; BCPT; 2,3-Bis(4-chlorophenyl)-5-phenyl-2H-tetrazol-3-ium chloride
EINECS	Contact for details

Quality Control

Our 2,3-Bis(4-Chlorophenyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment away from direct light exposure to maintain stability and potency.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.