Description

Mmp-1 Substrate I, Fluorogenic is a specialized fluorogenic peptide substrate designed for the sensitive and continuous detection of Matrix Metalloproteinase-1 (MMP-1, Collagenase-1) activity. This compound is critical for biochemical research and drug discovery, enabling the study of enzyme kinetics, inhibitor screening, and proteolytic activity in complex biological samples. It is an essential tool for researchers and scientists in pharmaceutical development, academic biochemistry, and diagnostic assay manufacturing focused on tissue remodeling, cancer metastasis, and inflammatory diseases.

Application

• High-Throughput Screening (HTS) for MMP-1 inhibitor discovery and development in pharmaceutical research.
• Enzyme Kinetic Studies to determine kinetic parameters (Km, Vmax, kcat) of MMP-1 activity.
• Biochemical Assay Development for monitoring MMP-1 activity in cell culture supernatants, tissue extracts, and biological fluids.
• Protease Activity Profiling in research related to cancer, arthritis, cardiovascular diseases, and other conditions involving extracellular matrix degradation.
• Diagnostic Kit Component for the development of in vitro diagnostic (IVD) assays measuring collagenase activity.
• Quality Control Testing in biopharmaceutical production where MMP activity needs to be monitored or controlled.

Basic Information

Product Name	Mmp-1 Substrate I, Fluorogenic
CAS No.	135662-04-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	MMP-1 Fluorogenic Substrate; Collagenase-1 Substrate; Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2; Fluorogenic Peptide Substrate for MMP-1; Matrix Metalloproteinase-1 Substrate I; (7-Methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-Ala-Arg-NH2; Dnp-Pro-Leu-Gly-Leu-Trp-Ala-Arg (common sequence reference); Mca-PLGL-Dpa-AR-NH2
EINECS	Contact for details

Quality Control

Our Mmp-1 Substrate I, Fluorogenic is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, correct structural identity, and optimal performance in enzymatic assays. Certificates of Analysis (COA) detailing purity (by HPLC), identity (by MS and/or NMR), and performance data are available upon request. We support research requiring consistent, reliable, and well-characterized biochemical tools.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥95% (Area Percent)
Water Content (KF)	≤5.0%
Functional Activity	Conforms to reference standard in MMP-1 assay
Solubility	Soluble in DMSO, DMF; Limited solubility in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.