Description

Fluorescein3,6-Diphosphate CAS NO 134869-03-7 is a high-purity, enzymatically cleavable derivative of fluorescein, serving as a critical substrate for phosphatase and other enzyme activity assays. Its core value lies in generating a highly fluorescent signal upon dephosphorylation, enabling sensitive and quantitative detection in biochemical research and diagnostic applications. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and in vitro diagnostics (IVD) sectors who require reliable, high-performance reagents for enzymatic studies and assay kits.

Application

• Substrate for Alkaline Phosphatase (ALP) and Acid Phosphatase (ACP) Assays: Widely used in ELISA, histochemistry, and microplate-based detection systems for research and clinical diagnostics.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for screening inhibitors or activators of phosphatase enzymes in drug target validation.
• Cell-Based Assays & Reporter Systems: Utilized to monitor intracellular phosphatase activity and signal transduction pathways in live or fixed cells.
• In Vitro Diagnostic (IVD) Reagent Manufacturing: A key component in the formulation of clinical chemistry reagents for automated analyzers measuring enzyme levels.
• Biomarker Detection & Research: Applied in developing sensitive assays for detecting disease-specific phosphatase biomarkers.
• Food & Environmental Safety Testing: Used in enzymatic methods for detecting contaminants or specific analytes that correlate with phosphatase activity.

Basic Information

Product Name	Fluorescein3,6-Diphosphate
CAS No.	134869-03-7
Molecular Formula	C20H14O13P2
Molecular Weight	548.26 g/mol
Synonyms	Fluorescein Diphosphate; FDP; 3',6'-Bis(phosphoryloxy)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; Fluorescein 3,6-Bisphosphate; Fluorescein 3,6-Diphosphoric Acid; Fluorescein-3,6-diphosphoric Acid Trisodium Salt (common salt form); Fluorogenic Phosphatase Substrate; ALP Substrate FDP
EINECS	Contact for details

Quality Control

Our Fluorescein3,6-Diphosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment. We can supply material compliant with the specifications required for GMP manufacturing of in vitro diagnostic devices upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate and keep frozen. Allow the sealed container to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0% (Anhydrous basis)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, DMSO, buffer solutions
Fluorescence Properties	Ex/Em ~490/514 nm (upon dephosphorylation)
Enzymatic Activity	Suitable for phosphatase assay development

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.