Description

Benzyloxycarbonylcitrullyl-Arginine 4-Methylcoumarin-7-Ylamide is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critically important for researchers and quality control laboratories requiring precise detection and quantification of protease activity, particularly for enzymes like thrombin and trypsin. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and the development of clinical assays.

Application

• Protease Activity Assays: A key fluorogenic substrate for the continuous, real-time measurement of serine protease activity, including thrombin, trypsin, and related enzymes.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: Essential for kinetic studies, enzyme characterization, and mechanistic investigations in academic and industrial research settings.
• Diagnostic Development: Serves as a critical component in developing sensitive in-vitro diagnostic (IVD) tests for conditions related to coagulation and other protease-mediated pathways.
• Quality Control (QC): Employed in pharmaceutical QC labs to monitor the purity and activity of enzyme-based biopharmaceuticals during manufacturing.

Basic Information

Product Name	Benzyloxycarbonylcitrullyl-Arginine 4-Methylcoumarin-7-Ylamide
CAS No.	134665-86-4
Molecular Formula	C₃₀H₃₈N₈O₈
Molecular Weight	638.67 g/mol
Synonyms	Z-Cit-Arg-AMC; Z-Cit-Arg-7-amido-4-methylcoumarin; Benzyloxycarbonyl-L-citrullyl-L-arginine 7-amido-4-methylcoumarin; Thrombin Substrate IV; Boc-Cit-Arg-AMC; Z-Cit-Arg-MCA; (S)-2-(((S)-1-((S)-5-((((Benzyloxy)carbonyl)amino)-6-((7-((amino(imino)methyl)amino)-4-methyl-2-oxo-2H-chromen-3-yl)amino)-6-oxohexyl)amino)-4-(carbamoylamino)-1-oxobutan-2-yl)carbamoyl)pyrrolidine-1-carboxylate
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylcitrullyl-Arginine 4-Methylcoumarin-7-Ylamide is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. All products undergo rigorous analytical testing, including HPLC for purity and identity confirmation via mass spectrometry. Certificates of Analysis (COA) detailing lot-specific results are available upon request to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may lead to degradation. For long-term stability, store desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.