Description

7-Hydroxycoumarin-3-Carboxylic Acid n-Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent. This compound is critical for creating stable, fluorescent conjugates with proteins, peptides, and other biomolecules containing primary amine groups. It is essential for researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors who require precise and sensitive detection methods.

Application

• Fluorescent Labeling of Biomolecules: Primary reagent for covalently attaching a fluorescent coumarin tag to antibodies, proteins, peptides, and oligonucleotides for detection and imaging.
• Bioconjugation & Probe Synthesis: Used in the synthesis of active fluorescent probes and sensors for enzymatic activity, receptor binding studies, and cellular trafficking.
• Diagnostic Assay Development: Key component in developing fluorescent immunoassays (FLISA), lateral flow tests, and other in-vitro diagnostic (IVD) kits.
• Cell Biology & Microscopy: Enables the labeling of cellular components for fluorescence microscopy, flow cytometry, and high-content screening applications.
• Pharmaceutical Research: Utilized in drug discovery for labeling target molecules to study drug-target interactions, pharmacokinetics, and biodistribution.
• Material Science Surface Modification: Functionalizes surfaces (e.g., nanoparticles, microarrays, polymers) with fluorescent groups for sensing and biointerface studies.

Basic Information

Product Name	7-Hydroxycoumarin-3-Carboxylic Acid n-Succinimidyl Ester
CAS No.	134471-24-2
Molecular Formula	C14H9NO7
Molecular Weight	303.23 g/mol
Synonyms	7-Hydroxycoumarin-3-carboxylic acid N-hydroxysuccinimide ester; 7-Hydroxy-3-carboxycoumarin NHS ester; Umbelliferone-3-carboxylic acid N-succinimidyl ester; Coumarin 120 NHS ester; 3-Carboxy-7-hydroxycoumarin NHS ester; 7-Hydroxy-3-coumarincarboxylic acid N-succinimidyl ester; 7-Hydroxy-3-carboxycoumarin, N-succinimidyl ester; Amine-reactive coumarin 120 derivative
EINECS	Contact for details

Quality Control

Our 7-Hydroxycoumarin-3-Carboxylic Acid n-Succinimidyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high reactivity. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) with detailed specifications are provided to guarantee the product meets the stringent requirements for sensitive bioconjugation and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.