Description

Theophylline, [8-3H] is a tritium-labeled derivative of the methylxanthine bronchodilator, theophylline. This radiolabeled compound is an essential tool for conducting advanced pharmacokinetic, metabolic, and receptor-binding studies with high sensitivity and specificity. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in respiratory drug development and biochemical research. The product, identified by CAS No. 134459-03-3, enables precise tracking and quantification in complex biological systems.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used as a radiotracer to investigate the absorption, distribution, metabolism, and excretion (ADME) profile of theophylline and related compounds.
• Receptor Binding Assays: Employed in competitive binding studies to characterize adenosine receptor interactions and screen for new bronchodilator candidates.
• Biochemical Pathway Analysis: Facilitates research into the mechanisms of action of methylxanthines, including phosphodiesterase inhibition and calcium mobilization.
• Analytical Method Development: Serves as an internal standard or reference material in the development and validation of sensitive LC-MS/MS or radio-HPLC methods.
• Preclinical Research: Critical for autoradiography studies and tissue distribution analysis in animal models of asthma and COPD.

Basic Information

Item	Detail
Product Name	Theophylline, [8-3H]
CAS No.	134459-03-3
Molecular Formula	C7H73H N4O2
Molecular Weight	~181.2 g/mol (exact mass depends on specific activity)
Synonyms	1,3-Dimethyl-3H-purine-2,6-dione, [8-3H]; 3H-Theophylline; [3H]-Theophylline; Theophylline-tritiated; 1,3-Dimethylxanthine, tritium labeled; Theo-d3; Radiolabeled Theophylline; TRC-Theophylline([8-3H])
EINECS	Contact for details

Quality Control

Every batch of Theophylline, [8-3H] is manufactured and analyzed under strict quality systems to ensure radiochemical purity, chemical purity, and specific activity meet the stringent requirements of research applications. Comprehensive quality control includes verification by HPLC with radiometric detection and NMR spectroscopy. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the exact specific activity (Ci/mmol or GBq/mmol), concentration, solvent, and analytical results.

Storage

Preserve in a tightly closed container, protected from light. Store the solution at or below -20°C in the original solvent as specified on the label. For long-term storage of dry material, keep in a desiccated environment at 2-8°C. Always follow appropriate radiation safety protocols and local regulations for handling and disposal of radioactive materials.

Specification

Item	Specification
Appearance	Clear colorless to slightly yellow solution (or solid)
Identification (HPLC)	Co-elutes with non-radiolabeled reference standard
Radiochemical Purity	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Specific Activity	10-30 Ci/mmol (370-1110 GBq/mmol) (Custom ranges available)
Concentration	1 mCi/mL (37 MBq/mL) in ethanol or as specified
Solvent	Ethanol, Methanol, or Aqueous Buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.