Description

Nicotinamide 8-Azidoadenine Dinucleotide is a chemically modified, photoactivatable analog of the essential coenzyme NAD+. This compound is a critical research tool designed for covalent labeling and cross-linking studies of NAD+-dependent enzymes and binding proteins. It is primarily utilized by scientists in the fields of biochemistry, enzymology, and structural biology for investigating protein-ligand interactions, mapping active sites, and developing affinity probes.

Application

• Enzyme Active Site Mapping: Used as a photoaffinity labeling reagent to covalently tag and identify the binding pockets of NAD+-dependent enzymes such as dehydrogenases, sirtuins, and PARPs.
• Protein Interaction Studies: Enables the study of protein-protein and protein-cofactor interactions through cross-linking experiments followed by mass spectrometry analysis.
• Affinity Probe Development: Serves as a key intermediate in the synthesis of biotinylated or fluorescently tagged NAD+ analogs for pull-down assays and detection.
• Structural Biology Research: Aids in the structural characterization of enzyme complexes by providing a mechanism for trapping transient interactions.
• Drug Discovery Screening: Employed in target identification and validation for therapeutic programs focused on NAD+-metabolizing pathways.
• Metabolic Pathway Analysis: Facilitates the study of NAD+ utilization and signaling in complex biological systems.

Basic Information

Product Name	Nicotinamide 8-Azidoadenine Dinucleotide
CAS No.	134388-69-5
Molecular Formula	C21H26N10O14P2
Molecular Weight	728.44 g/mol
Synonyms	8-Azido-NAD+; 8-N3-NAD+; 8-Azidoadenine NAD+; 8-Azido-nicotinamide adenine dinucleotide; Photoactivatable NAD+; NAD+ 8-azido analog; Azido-NAD; β-NAD+ 8-azido analog
EINECS	Contact for details

Quality Control

Our Nicotinamide 8-Azidoadenine Dinucleotide is manufactured under strict quality protocols suitable for research applications. Each batch is characterized to ensure identity, purity, and functional integrity. We provide comprehensive analytical data including HPLC purity, NMR, and MS analysis. Certificates of Analysis (COA) with detailed specifications are available upon request to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Aliquoting is recommended to minimize freeze-thaw cycles and exposure to atmospheric moisture. Handle in a controlled environment.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in water, buffer solutions
Functional Activity	Tested in standard enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.