Description

6-Chloro-3-Indoxyl Nonanoate is a specialized chromogenic substrate used in enzymatic detection and histochemical staining. This compound matters for its high sensitivity and specificity in revealing enzymatic activity, particularly for certain hydrolases, through the formation of an insoluble, colored precipitate. It is primarily needed by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in molecular biology, pathology, and biochemical assay development.

Application

• Enzymatic Substrate for Hydrolases: Used as a specific chromogenic substrate for detecting enzymatic activity, particularly for esterases and lipases, in biochemical research.
• Histochemistry and Cytochemistry: Employed in tissue staining protocols to localize specific enzyme activity within cells and tissue sections under a microscope.
• Diagnostic Assay Development: A key component in the formulation of diagnostic kits and reagents for clinical and research laboratories.
• Microbiology Research: Used in microbial identification and characterization studies based on specific enzymatic profiles.
• Biochemical Pathway Analysis: Aids in the study of metabolic pathways and enzyme kinetics in vitro.
• Pharmaceutical Screening: Utilized in high-throughput screening assays to identify potential enzyme inhibitors or activators for drug discovery.

Basic Information

Product Name	6-Chloro-3-Indoxyl Nonanoate
CAS No.	133950-72-8
Molecular Formula	C₁₇H₂₂ClNO₃
Molecular Weight	323.81 g/mol
Synonyms	6-Chloro-3-indolyl nonanoate; 6-Chloroindoxyl nonanoate; 3-Indoxyl, 6-chloro-, nonanoate; Nonanoic acid 6-chloro-1H-indol-3-yl ester; 6-Chloro-3-indoxyl nonanoic acid ester; 6-Cl-3-INON; Chromogenic substrate for esterase/lipase.
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indoxyl Nonanoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.