Description

5-Bromo-3-Indoxyl Nonanoate is a chromogenic enzyme substrate widely used in biochemical and diagnostic applications. This compound is valued for its role in detecting specific enzymatic activity through the release of an insoluble, colored indigo dye upon hydrolysis. It is primarily utilized by researchers and manufacturers in the life sciences, diagnostic kit production, and industrial enzyme testing sectors. The product is supplied to meet the stringent purity requirements of these advanced applications.

Application

• Diagnostic Reagent Manufacturing: A key substrate for the detection of esterase and lipase activity in clinical and veterinary diagnostic kits.
• Microbiological Research: Used in agar plate assays to identify and differentiate bacterial colonies based on specific enzyme profiles.
• Histochemistry & Cell Biology: Employed as a staining agent for the localization of enzymatic activity in tissue sections and cell cultures.
• Enzyme Characterization: Serves as a specific substrate in biochemical assays to study the kinetics and inhibition of target enzymes.
• Industrial Biotechnology: Applied in high-throughput screening (HTS) platforms for the discovery of novel enzymes or inhibitors.
• Quality Control Testing: Used within production facilities to verify the activity and consistency of enzyme batches.

Basic Information

Product Name	5-Bromo-3-Indoxyl Nonanoate
CAS No.	133950-70-6
Molecular Formula	C₁₇H₂₂BrNO₃
Molecular Weight	368.27 g/mol
Synonyms	5-Bromo-3-indolyl nonanoate; 3-Indolol, 5-bromo-, nonanoate; 5-Bromo-3-indoxyl nonanoate; Nonanoic acid 5-bromo-1H-indol-3-yl ester; 5-Br-3-IN; Bromoindoxyl nonanoate
EINECS	Contact for details

Quality Control

Our 5-Bromo-3-Indoxyl Nonanoate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.