Description

Endothelin Converting Enzyme Substrate CAS NO 133752-35-9 is a specialized biochemical reagent designed for research into the endothelin system, a critical pathway in cardiovascular regulation. This high-purity peptide substrate is essential for studying the enzymatic activity of Endothelin Converting Enzyme (ECE), which plays a pivotal role in the biosynthesis of potent vasoconstrictors. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and diagnostic developers focused on hypertension, pulmonary arterial hypertension, and related cardiovascular diseases.

Application

• Enzymatic Activity Assays: Serving as a specific substrate for in vitro studies of Endothelin Converting Enzyme (ECE-1) kinetics and inhibition.
• Drug Discovery & Screening: A key tool in high-throughput screening (HTS) campaigns to identify and characterize novel ECE inhibitors for cardiovascular therapeutics.
• Biochemical Research: Used to investigate the endothelin pathway's role in vascular tone, cell proliferation, and fibrosis mechanisms.
• Diagnostic Development: Employed in the development of clinical assays to measure ECE activity in biological samples.
• Academic & Institutional Research: Fundamental for university and research institute labs studying cardiovascular physiology and pathophysiology.
• Quality Control for Peptide Synthesis: Can be used as a reference standard in the quality assessment of related peptide compounds.

Basic Information

Product Name	Endothelin Converting Enzyme Substrate
CAS No.	133752-35-9
Molecular Formula	C46H68N12O13S2
Molecular Weight	1045.2 g/mol
Synonyms	Big Endothelin-1 (1-38), Human; Big ET-1 (1-38); Endothelin-1 Precursor (1-38); ET-1 (1-38); [Cys1, Cys15]-Big Endothelin-1 (Human); Preproendothelin-1 (1-38); ECE Substrate Peptide; H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-OH (Disulfide bond: 1-15)
EINECS	Contact for details

Quality Control

Our Endothelin Converting Enzyme Substrate is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is subjected to comprehensive analytical testing, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and amino acid analysis (AAA). A detailed Certificate of Analysis (COA) documenting purity, peptide content, and solvent residues is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Amino Acid Composition	Within ±10% of theoretical
Solvent Residues	Meets ICH guidelines
Water Content (K.F.)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.