Description

n-Succinyl-Ile-Ile-Trp 7-Amido-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound matters for researchers and quality control labs requiring precise, sensitive, and continuous measurement of enzymatic activity, particularly for proteases like chymotrypsin. It is primarily needed by the life sciences, pharmaceutical R&D, and biotechnology industries for applications in enzyme kinetics, inhibitor screening, and diagnostic assay development.

Application

• Protease Activity Assays: A standard fluorogenic substrate for the continuous kinetic measurement of chymotrypsin and related serine proteases.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery to identify and characterize novel protease inhibitors in drug development pipelines.
• Biochemical Research: Enables detailed study of enzyme mechanisms, specificity, and kinetics in academic and industrial research settings.
• Diagnostic Kit Development: Serves as a key component in developing sensitive fluorometric diagnostic tests for clinical and research use.
• Quality Control Testing: Employed in biomanufacturing to monitor the activity and consistency of enzyme batches.

Basic Information

Product Name	n-Succinyl-Ile-Ile-Trp 7-Amido-4-Methylcoumarin
CAS No.	133525-12-9
Molecular Formula	C37H45N5O8
Molecular Weight	687.79 g/mol
Synonyms	Succinyl-Ile-Ile-Trp-AMC; Suc-Ile-Ile-Trp-AMC; N-Succinyl-L-Isoleucyl-L-Isoleucyl-L-Tryptophan 7-Amido-4-Methylcoumarin; AMC Substrate Suc-Ile-Ile-Trp; Protease Substrate 133525-12-9; Fluorogenic Peptide Substrate for Chymotrypsin; Chymotrypsin Substrate Suc-Ile-Ile-Trp-AMC
EINECS	Contact for details

Quality Control

Our n-Succinyl-Ile-Ile-Trp 7-Amido-4-Methylcoumarin is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.