Description

2',3'-Dideoxycytidine, [2',3'-3H(N)]- is a tritium-labeled nucleoside analog, a critical tool compound for biochemical and pharmacological research. Its primary value lies in its use as a radiolabeled tracer for studying metabolic pathways, DNA/RNA synthesis inhibition, and the pharmacokinetics of antiviral and anticancer agents. This high-specific-activity material is essential for researchers in pharmaceutical development, molecular biology, and virology, particularly for investigating the mechanisms of nucleoside reverse transcriptase inhibitors.

Application

• Biochemical Tracer Studies: Used to track the incorporation and metabolism of dideoxycytidine analogs in cellular systems.
• Antiviral Research: Serves as a key radioligand in the study of HIV and other viral replication mechanisms, specifically for probing the action of nucleoside analogs.
• Pharmacokinetics & ADME: Enables precise quantification of drug absorption, distribution, metabolism, and excretion in preclinical models.
• Enzyme Assay Development: Utilized in assays for enzymes like reverse transcriptase and DNA polymerases to measure inhibition kinetics.
• Metabolic Pathway Analysis: Investigates the anabolic and catabolic pathways of nucleoside drugs within cells.
• Autoradiography: Provides a means for visualizing the localization of dideoxycytidine derivatives in tissue samples.

Basic Information

Product Name	2',3'-Dideoxycytidine, [2',3'-3H(N)]-
CAS No.	133525-08-3
Molecular Formula	C9H13N3O3 (3H)
Molecular Weight	~228.2 g/mol (varies with specific activity)
Synonyms	2',3'-Dideoxycytidine-2',3'-3H; ddCyd-3H; Tritiated 2',3'-Dideoxycytidine; [2',3'-3H]ddC; 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-4-amino-2(1H)-pyrimidinone-2',3'-3H; Zalcitabine-3H; Radiolabeled ddC
EINECS	Contact for details

Quality Control

Every batch of 2',3'-Dideoxycytidine, [2',3'-3H(N)]- is manufactured and analyzed under strict quality systems to ensure identity, purity, and specific activity. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request and provide detailed data including radiochemical purity, chemical purity, specific activity, and solvent composition.

Storage

Preserve in a tightly closed container, protected from light. Store frozen (-20°C) or as specified on the label or Certificate of Analysis. For long-term storage, keep at -70°C or below. The product is highly volatile due to the potential for radiolytic decomposition and tritium exchange; appropriate containment and laboratory ventilation are required. Store away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Colorless to pale yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol:Water (Specified on COA)
Concentration	1 mCi/mL (Nominal)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.