Description

Boc-Glu(Obzl)-Ala-Arg-Amc Hcl is a protected tetrapeptide derivative, specifically a fluorogenic substrate for proteolytic enzymes. This compound is a critical research tool for studying enzyme kinetics and specificity, particularly for serine and cysteine proteases. It is essential for researchers and manufacturers in the fields of biochemistry, pharmaceutical development, and proteomics who require high-purity, well-characterized substrates for assay development and drug discovery.

Application

• Enzyme Assay Development: As a fluorogenic substrate for the study and characterization of proteases, including trypsin, thrombin, and other serine proteases.
• Biochemical Research: Used in academic and industrial laboratories to investigate enzyme mechanisms, inhibition, and substrate specificity.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors as drug candidates.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for measuring protease activity in clinical and research samples.
• Proteomics Studies: Utilized to analyze proteolytic pathways and protein degradation processes in complex biological systems.

Basic Information

Product Name	Boc-Glu(Obzl)-Ala-Arg-Amc Hcl
CAS No.	133448-25-6
Molecular Formula	C36H50N8O9 • HCl
Molecular Weight	775.30 g/mol (free base)
Synonyms	Boc-Glu(OBzl)-Ala-Arg-AMC hydrochloride; t-Butyloxycarbonyl-γ-benzyl-L-glutamyl-L-alanyl-L-arginine 7-amido-4-methylcoumarin hydrochloride; Boc-Glu(OBzl)-Ala-Arg-AMC • HCl; Boc-Glu(OBzl)-Ala-Arg-MCA HCl; Protease Substrate IV (AMC); Fluorogenic Protease Substrate; L-Glutamic acid, N-[(1,1-dimethylethoxy)carbonyl]-5-O-(phenylmethyl)-, L-alanyl-L-arginyl-7-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)amide, hydrochloride
EINECS	Contact for details

Quality Control

Our Boc-Glu(Obzl)-Ala-Arg-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.