Description

n-tert-Boc-Gly-Arg-Arg 7-Amido-4-Methylcoumarin HCl is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound matters because it provides a critical tool for biochemical research and pharmaceutical development, enabling precise enzymatic studies. It is primarily needed by researchers and quality control laboratories in the life sciences, pharmaceutical, and biotechnology industries for applications in drug discovery and diagnostic assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for trypsin-like serine proteases, including thrombin, plasmin, and factor Xa.
• Drug Discovery Screening: High-throughput screening (HTS) of potential protease inhibitors for therapeutic development.
• Biochemical Research: Kinetic studies and characterization of enzyme specificity and mechanism of action.
• Diagnostic Development: A key component in the development of fluorometric diagnostic kits for coagulation disorders or other protease-related pathologies.
• Cell Biology Studies: Monitoring protease activity in cellular and tissue extracts.
• Quality Control: Used in biopharmaceutical manufacturing to monitor and validate the activity of recombinant proteases.

Basic Information

Product Name	n-tert-Boc-Gly-Arg-Arg 7-Amido-4-Methylcoumarin HCl
CAS No.	133448-24-5
Molecular Formula	C34H53N11O9 • HCl
Molecular Weight	788.32 g/mol (free base)
Synonyms	Boc-Gly-Arg-Arg-AMC • HCl; Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin hydrochloride; t-Butyloxycarbonyl-Glycyl-L-arginyl-L-arginine 4-methyl-7-coumarylamide hydrochloride; Gly-Arg-Arg-AMC, Boc-; Thrombin Substrate I, Fluorogenic; Protease Substrate Boc-GRR-AMC; AMC Substrate Boc-GRR; t-Boc-Gly-Arg-Arg-AMC • HCl
EINECS	Contact for details

Quality Control

Our n-tert-Boc-Gly-Arg-Arg 7-Amido-4-Methylcoumarin HCl is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing specific results is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate at low temperature. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.