Description

n-T-Boc-Gly-Lys-Arg 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound matters for researchers and quality control labs requiring precise, real-time measurement of protease activity, particularly for enzymes like trypsin and thrombin. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, sensitive detection of trypsin, thrombin, and related serine proteases.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors.
• Biochemical Research: For kinetic studies, enzyme characterization, and determining kinetic parameters (Km, Vmax) of proteolytic enzymes.
• Diagnostic Development: A key component in developing in-vitro diagnostic tests for conditions related to abnormal protease activity.
• Quality Control in Biologics: Monitoring the activity and stability of enzyme-based therapeutics during manufacturing and storage.

Basic Information

Product Name	n-T-Boc-Gly-Lys-Arg 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	133448-23-4
Molecular Formula	C₃₃H₅₂N₁₀O₉ • HCl
Molecular Weight	753.30 g/mol (free base)
Synonyms	Boc-Gly-Lys-Arg-AMC • HCl; Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin hydrochloride; t-Boc-Gly-Lys-Arg-AMC; Fluorogenic Substrate for Trypsin; AMC Substrate Boc-Gly-Lys-Arg; N-α-t-Butyloxycarbonyl-glycyl-lysyl-arginine 7-amido-4-methylcoumarin hydrochloride; Trypsin Substrate I (Fluorogenic)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and diagnostic use. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) with detailed chromatographic data and spectroscopic profiles are available upon request, supporting your regulatory and quality assurance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. This compound is hygroscopic (moisture-sensitive). Allow the product to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.