Description

Boc-Glu(Obzl)-Gly-Arg-Amc Hcl is a protected tetrapeptide derivative, specifically a fluorogenic substrate, featuring a C-terminal 7-amino-4-methylcoumarin (AMC) group. This compound is of significant value in biochemical research and pharmaceutical development for studying protease activity and enzyme kinetics. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in protease inhibitor screening and mechanistic studies.

Application

• Protease Activity Assays: Used as a specific fluorogenic substrate for monitoring the activity of various proteolytic enzymes, including trypsin-like serine proteases.
• Enzyme Kinetic Studies: Essential for determining kinetic parameters (Km, Vmax, kcat) in fundamental biochemical research.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for screening libraries of compounds to identify novel protease inhibitors.
• Diagnostic Development: Serves as a key reagent in the development of clinical diagnostic tests that measure specific protease levels.
• Biochemical Pathway Analysis: A tool for investigating the role of specific proteases in cellular processes and disease pathways.
• Quality Control in Biologics: Can be utilized in QC labs to test for residual protease activity in biopharmaceutical products.

Basic Information

Product Name	Boc-Glu(Obzl)-Gly-Arg-Amc Hcl
CAS No.	133448-22-3
Molecular Formula	C₃₃H₄₄N₈O₉ • HCl
Molecular Weight	721.21 g/mol (free base)
Synonyms	Boc-Glu(OBzl)-Gly-Arg-AMC hydrochloride; t-Butyloxycarbonyl-γ-benzyl-L-glutamyl-glycyl-L-arginyl-7-amido-4-methylcoumarin hydrochloride; Boc-Glu(OBzl)-Gly-Arg-AMC • HCl; Boc-E(OBzl)-G-R-AMC hydrochloride; Fluorogenic Substrate for Proteases; Protease Substrate Boc-E(OBzl)GR-AMC; Nα-(tert-Butoxycarbonyl)-γ-benzyl-L-glutamyl-glycyl-L-arginyl-4-methyl-7-coumarylamide hydrochloride
EINECS	Contact for details

Quality Control

Our Boc-Glu(Obzl)-Gly-Arg-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥95.0%
Assay (HPLC)	≥98.0%
Water Content (KF)	≤2.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.