Description

Boc-Gln-Gly-Arg-Amc Hcl CAS NO 133448-21-2 is a protected tetrapeptide substrate featuring a C-terminal 7-amino-4-methylcoumarin (AMC) fluorophore. This compound is specifically designed for high-sensitivity enzymatic assays, serving as a critical tool for researchers studying protease activity and kinetics. It is essential for pharmaceutical R&D, biochemical research, and diagnostic kit development where precise measurement of enzymatic cleavage is required.

Application

• Protease Activity Assays: Primary substrate for fluorometric detection of trypsin-like serine proteases, including thrombin, plasmin, and factor Xa.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: A key reagent for studying enzyme kinetics, specificity, and catalytic mechanisms in purified systems.
• Diagnostic Development: Employed in the development of sensitive clinical diagnostic assays for conditions related to coagulation and fibrinolysis.
• Quality Control in Biologics: Used to monitor and quantify protease activity during the manufacturing and purification of biopharmaceuticals.

Basic Information

Product Name	Boc-Gln-Gly-Arg-Amc Hcl
CAS No.	133448-21-2
Molecular Formula	C29H44N10O8 • HCl
Molecular Weight	689.15 g/mol (Free base: 652.72 g/mol)
Synonyms	Boc-Gln-Gly-Arg-AMC hydrochloride; t-Butyloxycarbonyl-Glutamine-Glycine-Arginine-7-Amino-4-Methylcoumarin hydrochloride; Boc-Q-G-R-AMC • HCl; Fluorogenic Substrate for Trypsin-like Proteases; Boc-Gln-Gly-Arg-AMC; 7-Amino-4-methylcoumarin labeled tetrapeptide; Protease Substrate V; Thrombin Substrate I (AMC)
EINECS	Contact for details

Quality Control

Every batch of Boc-Gln-Gly-Arg-Amc Hcl is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high purity and batch-to-batch consistency suitable for sensitive research applications. A detailed Certificate of Analysis (COA) providing results for identity, purity (HPLC), and specific tests is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.