Description

Ac-Ala-Ala-Pro-Val-Pna CAS NO 133261-53-7 is a synthetic, chromogenic peptide substrate designed for the specific detection and quantification of enzymatic activity. This compound is a critical reagent in biochemical research and diagnostic development, offering high sensitivity and specificity for target proteases. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and assay development.

Application

• Enzyme Activity Assays: Used as a specific chromogenic substrate for measuring the activity of target proteases, such as dipeptidyl peptidases and related enzymes.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential enzyme inhibitors for therapeutic development.
• Biochemical Research: Serves as a key tool for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial laboratories.
• Diagnostic Kit Development: Integrated into the formulation of diagnostic test kits for clinical and research use, enabling the detection of specific enzymatic biomarkers.
• Quality Control in Bioprocessing: Applied in monitoring enzymatic processes and ensuring consistency in the production of biologics and other protein-based products.

Basic Information

Product Name	Ac-Ala-Ala-Pro-Val-Pna
CAS No.	133261-53-7
Molecular Formula	C24H34N6O7
Molecular Weight	518.57 g/mol
Synonyms	Acetyl-Ala-Ala-Pro-Val-p-Nitroanilide; Ac-AAPV-pNA; N-Acetyl-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide; Acetyl-Alanyl-Alanyl-Prolyl-Valyl-4-Nitroanilide; AAPV-pNA; DPP IV Substrate; Dipeptidyl Peptidase IV Substrate; Chromogenic Peptide Substrate AAPV-pNA
EINECS	Contact for details

Quality Control

Every batch of Ac-Ala-Ala-Pro-Val-Pna is subjected to rigorous analytical testing to ensure identity, purity, and performance consistency. Our quality assurance protocols are designed to meet the stringent requirements of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identity confirmation, and other critical parameters is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Extinction Coefficient	~8,800 M-1cm-1 at 405 nm (in assay buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.