Description

1H-Thieno[3,4-D]Imidazole-4-Pentanamide, n-[5-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-5-Oxopentyl]Hexahydro-2-Oxo-, (3As,4S,6Ar)- is a high-purity, advanced pharmaceutical intermediate featuring a reactive N-hydroxysuccinimide (NHS) ester group. This compound is specifically engineered for bioconjugation and linker chemistry, enabling the stable attachment of molecules to proteins, peptides, and other amine-containing substrates. It is an essential building block for researchers and manufacturers in biopharmaceutical development, diagnostic assay production, and advanced drug delivery systems.

Application

• Bioconjugation Reagent: Primary use as an amine-reactive crosslinker for coupling peptides, drugs, or labels to antibodies, proteins, and nanoparticles.
• Antibody-Drug Conjugate (ADC) Development: Serves as a critical linker component in the synthesis of targeted cancer therapeutics.
• Diagnostic Reagents: Used to create enzyme conjugates and labeled probes for immunoassays (ELISA, lateral flow) and histochemistry.
• Biomaterial Functionalization: Modification of polymer surfaces, hydrogels, and medical devices to introduce bioactive motifs.
• Peptide & Protein Modification: Site-specific labeling for fluorescence, biotinylation, or PEGylation to study structure, function, and interactions.
• Solid-Phase Synthesis: Employed in the preparation of custom affinity resins and immobilized ligands for chromatography.

Basic Information

Product Name	1H-Thieno[3,4-D]Imidazole-4-Pentanamide, n-[5-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-5-Oxopentyl]Hexahydro-2-Oxo-, (3As,4S,6Ar)-
CAS No.	133117-37-0
Molecular Formula	C20H28N4O7S
Molecular Weight	468.52 g/mol
Synonyms	Biotin-PEG4-NHS Ester; Biotinamidohexanoyl-PEG4-NHS; Biotin-PEG4-NHS; Biotin-(PEG)4-NHS ester; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(5-((2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)pentanamide; Biotinamidocaproyl-PEG4-NHS; LC-Biotin-PEG4-NHS; EZ-Link™ Biotin-PEG4-NHS Ester (analog)
EINECS	Contact for details

Quality Control

Our production of this high-value intermediate adheres to stringent GMP-like guidelines to ensure batch-to-batch consistency and purity suitable for sensitive research and development applications. Identity, purity, and impurity profiles are verified using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active NHS ester.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.