Description

H-Lys-Pro-Amc CAS NO 133066-53-2 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of specific protease activity. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic applications where accurate enzyme kinetics are paramount.

Application

• Biochemical Research: Used as a selective substrate for studying the kinetics and inhibition of specific proteases, such as dipeptidyl peptidases.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize potential therapeutic enzyme inhibitors.
• Diagnostic Development: Serves as a key component in developing fluorogenic assays for clinical diagnostics and biomarker detection.
• Enzyme Characterization: Essential for determining the specificity and catalytic efficiency of purified enzymes in vitro.
• Cell Biology Studies: Applied in live-cell imaging and flow cytometry to monitor intracellular protease activity.
• Quality Control (QC): Used as a reference standard in QC laboratories for validating assay performance and reagent consistency.

Basic Information

Product Name	H-Lys-Pro-Amc
CAS No.	133066-53-2
Molecular Formula	C20H29N5O4
Molecular Weight	403.48 g/mol
Synonyms	Lys-Pro-AMC; H-Lys-Pro-7-Amino-4-methylcoumarin; L-Lysyl-L-proline 7-amido-4-methylcoumarin; DPP IV Substrate; DPP-4 Substrate; Dipeptidyl Peptidase IV Fluorogenic Substrate; Lys-Pro-AMC Hydrochloride (if applicable); Substrate for DPP-4/DPP IV
EINECS	Contact for details

Quality Control

Every batch of H-Lys-Pro-Amc is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance. Our standard quality commitment includes comprehensive analysis by HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing batch-specific results are provided to guarantee compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference in standard enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.