Description

4-Nitrophenyl2-Acetamido-2-Deoxy-3,6-Di-o-Benzoyl-B-D-Galactopyranoside is a specialized glycoside derivative, primarily valued as a chromogenic substrate for enzymatic assays. This compound is critical for researchers and manufacturers requiring precise, colorimetric detection of specific glycosidase enzyme activity. It is essential for biochemical research, pharmaceutical development, and diagnostic kit production where high-purity, reliable substrates are non-negotiable.

Application

• Enzymatic Substrate for Glycosidases: Acts as a chromogenic substrate for β-galactosidase and related enzymes, producing a measurable color change upon cleavage.
• Biochemical Research & Assay Development: Used in kinetic studies, enzyme characterization, and inhibitor screening in academic and industrial laboratories.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical and research purposes, including genetic and metabolic disorder testing.
• Pharmaceutical Intermediate: Serves as a synthetic intermediate or reference standard in the development of carbohydrate-based therapeutics and glycobiology studies.
• Quality Control in Biotechnology: Employed for the validation and quality control of enzyme preparations and recombinant protein products.

Basic Information

Product Name	4-Nitrophenyl2-Acetamido-2-Deoxy-3,6-Di-o-Benzoyl-B-D-Galactopyranoside
CAS No.	132498-72-7
Molecular Formula	C28H24N2O10
Molecular Weight	548.50 g/mol
Synonyms	p-Nitrophenyl N-acetyl-3,6-di-O-benzoyl-β-D-galactopyranoside; 4-Nitrophenyl 2-acetamido-2-deoxy-3,6-di-O-benzoyl-β-D-galactopyranoside; pNP-β-D-GalNAc(3,6-di-O-Bz); GalNAc-β-pNP (3,6-di-O-benzoylated); β-D-Galactopyranoside, 2-(acetylamino)-2-deoxy-3,6-bis-O-(phenylcarbonyl)-, 4-nitrophenyl; Chromogenic β-galactosidase substrate (benzoylated); 2-Acetamido-2-deoxy-3,6-di-O-benzoyl-β-D-galactopyranosyl 4-nitrophenyl ether
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl2-Acetamido-2-Deoxy-3,6-Di-o-Benzoyl-B-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for research chemicals, ensuring reliable performance as a critical assay component.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.