Description

P(1)-N(6)-(4-Azidophenylethyl)Adenosine-P(2)-(4-(3-Azidopyridinio)Butyl)Diphosphate is a specialized, bifunctional photoactivatable crosslinking agent derived from adenosine nucleotides. This compound is engineered for advanced biochemical research, enabling the covalent capture and identification of transient protein-ligand and protein-protein interactions. It is primarily utilized by research scientists and process developers in the fields of structural biology, enzymology, and drug discovery to map binding sites and study molecular recognition events.

Application

• Photoaffinity Labeling (PAL): A critical tool for identifying and characterizing the binding sites of proteins, enzymes, and receptors.
• Structural Biology Studies: Used to probe the three-dimensional architecture of nucleotide-binding pockets and active sites.
• Enzyme Mechanism Analysis: Employed to trap and study intermediate states in enzymatic catalysis involving ATP or related nucleotides.
• Drug Discovery & Target Validation: Facilitates the identification of novel drug targets by covalently labeling proteins that interact with nucleotide analogs.
• Proteomics Research: Enables the crosslinking and subsequent isolation of protein complexes for mass spectrometric analysis.
• Bioconjugation & Probe Development: Serves as a key intermediate for synthesizing advanced diagnostic or research probes.

Basic Information

Product Name	P(1)-N(6)-(4-Azidophenylethyl)Adenosine-P(2)-(4-(3-Azidopyridinio)Butyl)Diphosphate
CAS No.	132210-01-6
Molecular Formula	C₂₇H₃₄N₁₁O₁₂P₂
Molecular Weight	790.59 g/mol
Synonyms	N6-[2-(4-Azidophenyl)ethyl]-2'-deoxy-2'-adenosine-5'-triphosphate; 8-Azido-ATP analog; Bifunctional Azido-nucleotide Crosslinker; Photoactivatable Adenosine Diphosphate Derivative; 5'-O-[{4-[3-Azidopyridinio]butyl}hydroxyphosphinyl]-N6-[2-(4-azidophenyl)ethyl]adenosine Diphosphate; PAL Probe for ATP-binding proteins; Azidophenylethyl-azidopyridinio-ADP
EINECS	Contact for details

Quality Control

Every batch of this high-purity biochemical is subjected to rigorous analytical characterization to ensure identity, purity, and functional integrity for sensitive research applications. Standard quality protocols include HPLC for purity assessment and spectroscopic methods for structural confirmation. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to support your research documentation and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, Methanol, Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.