Description

n-Blocked-5'-o-Dmt-2-o-Fpmp Ced Inosine Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer, a critical building block for the solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of modified inosine bases into synthetic DNA and RNA strands, which is essential for advanced research and therapeutic development. It is primarily needed by biotechnology and pharmaceutical companies engaged in antisense drug discovery, aptamer development, and fundamental nucleic acid research where specific base modifications are required.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for the automated synthesis of DNA/RNA strands containing inosine residues.
• Antisense and siRNA Therapeutics: For the research and development of oligonucleotide-based drugs where inosine modifications can alter binding affinity and specificity.
• Aptamer Development: In the creation of synthetic nucleic acid ligands (aptamers) for diagnostic and therapeutic targeting.
• PCR Primers and Probes: For designing primers and probes with degenerate bases or reduced melting temperatures.
• Structural Biology Studies: To investigate nucleic acid-protein interactions and the role of specific base pairing.
• Synthesis of Modified Antisense Oligonucleotides (ASOs): For developing next-generation ASO therapeutics with improved pharmacokinetic properties.

Basic Information

Product Name	n-Blocked-5'-o-Dmt-2-o-Fpmp Ced Inosine Phosphoramidite
CAS No.	131541-04-3
Molecular Formula	C₄₆H₅₅FN₅O₈P
Molecular Weight	879.93 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(R)-1-(2-fluorophenyl)-4-methoxypiperidin-4-yl]-N2-phenoxyacetyl-inosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Fpmp-5'-O-DMT-N-Pac-Inosine Phosphoramidite; Inosine, 2'-O-[(1R)-1-(2-fluorophenyl)-4-methoxypiperidin-4-yl]-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N-[2-(phenoxy)acetyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 2'-O-[(R)-1-(2-Fluorophenyl)-4-methoxypiperidin-4-yl]-5'-O-DMT-N-Pac-inosine-3'-CE phosphoramidite; Fpmp-protected Inosine Phosphoramidite
EINECS	Contact for details

Quality Control

Our n-Blocked-5'-o-Dmt-2-o-Fpmp Ced Inosine Phosphoramidite is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive oligonucleotide synthesis. Each lot is rigorously tested via HPLC, ¹H/³¹P NMR, and mass spectrometry to confirm identity, purity, and reactivity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (³¹P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.