Description

D-Luciferin-6-o-β-D-Galactopyranoside is a specialized, enzymatically cleavable substrate for firefly luciferase, designed for advanced bioluminescent assays. This compound matters because it enables highly sensitive, real-time detection and quantification of β-galactosidase activity in live cells and in vitro systems, serving as a critical tool for gene expression studies and high-throughput screening. It is essential for researchers and developers in the fields of molecular biology, drug discovery, and diagnostic assay development who require precise and reliable reporter gene analysis.

Application

• Reporter Gene Assays: Primary substrate for detecting and quantifying β-galactosidase reporter gene expression in mammalian cells.
• High-Throughput Screening (HTS): Enables luminescence-based screening of compound libraries for modulators of gene expression or enzyme activity in drug discovery.
• Cell-Based Imaging: Used in bioluminescence imaging (BLI) to monitor spatial and temporal gene expression patterns in live animals and tissue cultures.
• Diagnostic Development: Serves as a key component in the development of sensitive diagnostic kits for bacterial detection (e.g., E. coli) via β-galactosidase activity.
• Enzyme Kinetics Studies: Employed in biochemical assays to study the kinetics and inhibition of β-galactosidase enzyme.
• Signal Amplification Systems: Integrated into dual-reporter or cascading enzyme systems for amplified signal output in sensitive detection methodologies.

Basic Information

Product Name	D-Luciferin-6-o-β-D-Galactopyranoside
CAS No.	131474-38-9
Molecular Formula	C20H28N2O11S2
Molecular Weight	536.57 g/mol
Synonyms	D-Luciferin 6-O-β-D-galactopyranoside; β-Galactosidase Substrate (Luciferin derivative); 6-O-β-D-Galactopyranosyl-D-luciferin; D-Luciferin galactopyranoside; Galacton-Plus® (similar trade product); β-Galactosidase Luciferin Substrate; Luciferin-β-D-galactoside; D-Luc-β-D-Gal
EINECS	Contact for details

Quality Control

Our D-Luciferin-6-o-β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Bioluminescent Activity	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.