Description

D-(+)Biotin 2-Nitrophenyl Ester is a chemically activated derivative of biotin, a vital coenzyme in metabolic processes. This compound is specifically designed for bioconjugation, enabling the efficient labeling of biomolecules for detection and purification. It is an essential reagent for researchers and manufacturers in the pharmaceutical, diagnostic, and life science research sectors, facilitating advanced studies in molecular biology and the development of targeted therapeutics.

Application

• Bioconjugation Reagent: Primary use for covalently attaching biotin tags to proteins, antibodies, nucleic acids, and other biomolecules.
• Diagnostic Kit Development: Key component in the manufacture of ELISA, lateral flow assays, and other immunoassays that utilize streptavidin-biotin technology.
• Affinity Purification: Used to create biotinylated ligands for the isolation of specific targets via streptavidin or avidin matrices.
• Drug Discovery & Development: Employed in high-throughput screening (HTS) and target identification by labeling candidate molecules.
• Cell Biology & Imaging: Facilitates the study of cellular processes by labeling cell surface receptors or intracellular components for visualization.
• Proteomics Research: A tool for labeling and enriching proteins in complex mixtures for mass spectrometry analysis.
• Material Science: Used to functionalize surfaces and nanoparticles with biotin for biosensor and nanotechnology applications.

Basic Information

Item	Details
Product Name	D-(+)Biotin 2-Nitrophenyl Ester
CAS No.	131303-71-4
Molecular Formula	C16H17N3O5S
Molecular Weight	363.39 g/mol
Synonyms	Biotin 4-nitrophenyl ester; (+)-Biotin p-nitrophenyl ester; D-Biotin p-nitrophenyl ester; Biotin-PNP; Biotin 4-NPE; Vitamin H 2-nitrophenyl ester; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid 4-nitrophenyl ester
EINECS	Contact for details

Quality Control

Our D-(+)Biotin 2-Nitrophenyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, IR and NMR for structural confirmation, and specific tests for residual solvents and moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.