Description

4-Nitrophenyl o-(2- Acetamido-2-Deoxyglucopyranosyl)-(1-2)-o-(4-o-Methylmannopyranosyl)-(1-6)-Glucopyranoside is a specialized synthetic oligosaccharide derivative, serving as a critical chromogenic substrate for enzymatic assays. Its value lies in its precise structure, which makes it an essential tool for researchers studying glycosidase and glycosyltransferase activity. This high-purity biochemical is primarily needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions focused on carbohydrate chemistry and enzymology.

Application

• Enzymatic Substrate: Acts as a chromogenic substrate for the specific detection and kinetic analysis of α-glucosidase and related enzymes.
• Biochemical Research: Used in fundamental studies of carbohydrate metabolism, enzyme mechanisms, and inhibitor screening.
• Diagnostic Development: Employed in the formulation of diagnostic assays and kits for metabolic disorders like Pompe disease or diabetes research.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential therapeutic enzyme inhibitors.
• Quality Control: Serves as a reference standard in the quality control of enzyme preparations and diagnostic reagents.

Basic Information

Product Name	4-Nitrophenyl o-(2- Acetamido-2-Deoxyglucopyranosyl)-(1-2)-o-(4-o-Methylmannopyranosyl)-(1-6)-Glucopyranoside
CAS No.	131089-46-8
Molecular Formula	C33H50N2O22
Molecular Weight	826.76 g/mol
Synonyms	4-Nitrophenyl α-isomaltotrioside derivative; p-Nitrophenyl-α-D-Glcp-(1→2)-α-D-Glcp-(1→6)-α-D-Glcp-(1→?)-O-CH2-CH3 (structural variant); Chromogenic α-glucosidase substrate; Synthetic trisaccharide 4-NP derivative; PNPG derivative oligosaccharide; α-Glucosidase substrate for Pompe disease assay; 4-NP-α-isomaltotrioside
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl oligosaccharide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification (NMR, MS), to ensure it meets the exacting standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to maintain stability and prevent hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Biological Activity	Suitable for enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.