Description

Pglu-Pro-Arg-Mna is a synthetic tripeptide derivative containing a pyroglutamic acid (Pglu) residue and a 4-methoxy-2-naphthylamide (Mna) fluorogenic group. This compound serves as a highly specific and sensitive chromogenic substrate for the detection and quantification of thrombin and related serine proteases. It is an essential biochemical reagent for researchers and manufacturers in the diagnostics, pharmaceutical research, and life science sectors, enabling precise enzymatic activity studies and assay development.

Application

• Diagnostic Assay Development: As a key chromogenic substrate in clinical chemistry for measuring thrombin activity in coagulation testing.
• Pharmaceutical Research & Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate novel anticoagulant and antithrombotic drug candidates.
• Enzyme Kinetics & Biochemistry: For detailed kinetic studies of thrombin, factor Xa, and other trypsin-like serine proteases in academic and industrial R&D.
• Quality Control in Biologics: Employed in the potency testing and lot-release of therapeutic proteins that interact with the coagulation cascade.
• Hematology & Coagulation Monitoring: Integral to specialized reagents for automated analyzers in clinical laboratories to assess blood clotting function.

Basic Information

Product Name	Pglu-Pro-Arg-Mna
CAS No.	130835-45-9
Molecular Formula	C27H36N6O7
Molecular Weight	556.62 g/mol
Synonyms	Pyroglutamyl-Prolyl-Arginyl-4-Methoxy-2-Naphthylamide; H-pGlu-Pro-Arg-MNA; L-Pyroglutamyl-L-prolyl-L-arginine 4-methoxy-2-naphthylamide; Chromogenic Substrate for Thrombin; Thrombin Substrate I (MNA-based); BOCHEM BG 0025; Benzyloxycarbonyl-Gly-Pro-Arg-4-methoxy-2-naphthylamide (related compound)
EINECS	Contact for details

Quality Control

Our Pglu-Pro-Arg-Mna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and specific activity validation in enzymatic assays. We provide full traceability and Certificates of Analysis (COA) to support your regulatory and research requirements, ensuring consistent performance and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Enzymatic Activity	Tested for functionality with thrombin

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.